Study on Screening of Chlamydospore Formation Mutants from T-DNA Insertional Mutant Library of Trichoderma harzianum and Cloning of T-DNA Tagging Sequence

doi:10.7668/hbnxb.2009.05.008

Abstract

Abstract: 7 mutants with chlamydospore format ion differences were selected from 1 192 transformants cultured in PD liquid medium.The conidia formation ability of all these 7 mutants was signif icantly lower than that of the wild type T.harzianum.The characterization of these 7 mutants was analyzed.The results showed that as for the mutants 918,1137,1317 chlamydospore developed in the mycelium and falled off from mycelium when incubated time prolonged.However,as for the other 4 mutants 627,840,1137,1173 the chlamydospore couldn't falled off from mycelium when incubated time prolonged.Using TAIL-PCR to clone T-DNA right border sequences from spore formation mutants,we successfully obtained the fungal genomic DNA sequences flanking T-DNA right border from 5 transformants.The homologous sequences of 3 sequences were found by using Blastn and Blastx from NCBI GenBank.However,the homologous sequences of other 2 sequences were not found.They might be new genes related to spore formation which need to be further studied.This study is the base for the cloning of chlamydospore related gene and the mechanism research of chlamydospore formation.

Key words: Trichoderma harzianum, T-DNA, Mutant, TAIL-PCR, Chlamydospore

CLC Number:

Q933

HUANG Ya-li, JIANG Xi-liang, TIAN Yun-long, ZHU Chang-xiong. Study on Screening of Chlamydospore Formation Mutants from T-DNA Insertional Mutant Library of Trichoderma harzianum and Cloning of T-DNA Tagging Sequence[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 35-39. doi: 10.7668/hbnxb.2009.05.008.

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