Screening of Mutants Susceptible to Puccina triticina in TcLr19 Induced by EMS

doi:10.7668/hbnxb.2016.04.015

Abstract

Abstract: In order to study the function and mechanism of the resistance gene of wheat leaf rust,seeds of wheat TcLr19 was treated by EMS.The leaf rust resistance and agronomic traits were investigated and phenotypic mutants were identified in M₁ and M₂.A total of 367 individual plants were obtained in M₁ with different concentrations of EMS.According to the emergence rate,seed rate and mutation rate,EMS 1.0% was considered the optimum concentration.In 2 359 M₂ mutant,a total of 38 phenotype mutations were screened out with 1.61% mutation rate,and a total of 53 susceptible mutations to Puccinia triticina were screened out with 2.25% mutation rate.A total of 146 susceptible plants were obtained in 459 M₃ susceptible populations,especially M₃6-2,M₃33-8,M₃33-9,M₃33-11,M₃44-4 and M₃96-8,and the frequency of susceptible mutation were more than 70%.Furthermore,Lr19 molecular marker-assisted selection was also used in order to guarantee the reliability of the results in the test.The results showed that EMS treatment was an effective method in screening of mutants susceptible to P.triticina.The result not only provided important genetic resources for wheat leaf rust resistance gene cloning and functional genomic research,but also provided new germplasms in wheat breeding.

Key words: Wheat, Leaf rust, Ethyl methane sulfonate (EMS), Mutant

CLC Number:

ZHANG Weihong, AN Zhe, FAN Xuefeng, FENG Yueqi, YANG Wenxiang, LIU Daqun. Screening of Mutants Susceptible to Puccina triticina in TcLr19 Induced by EMS[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(4): 88-93. doi: 10.7668/hbnxb.2016.04.015.

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