Construction and Phenotype Analysis of CPK10/CPK30 Double Mutantin Arabidopsis

doi:10.7668/hbnxb.2016.04.016

Abstract

Abstract: Calcium-dependent protein kinases (CDPKs) function as Ca²⁺ sensor in various plant physiological processes.CPK10 was one member of CDPK family.In order to investigate the molecular mechanisms of CPK10 and it's high homology CPK30 in response to stress,cpk10×cpk30 double mutant was firstly constructed.The phenotypes in response to various stress were detected,and the RT-PCR method was used to analyzed the two genes expression.The results showed that there was no difference in the seed germination stage between double mutant and wild-type plants in the treatment of drought,salt and ABA.In the mature stage,double mutant exhibited no difference to drought stress compared with wild-type and single mutant.Nevertheless,the expression levels of RD29A was decreased in the double mutant and showed opposite trend in wild-type and single mutant during drought stress.The expression of ABA-responsive gene OST1 was enhanced obviously in double mutant after drought stress 0.5 h.The cpk10×cpk30 double mutant was successfully obtained.Our data indicated that CPK10 and CPK30 might be involved in ABA-dependent signal transduction pathway,and showed redundant functions.

Key words: CDPK, Double mutant, Adversity stress, Phenotype analysis

CLC Number:

XIE Xin, SUN Ning, WEI Fengju. Construction and Phenotype Analysis of CPK10/CPK30 Double Mutantin Arabidopsis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(4): 94-99. doi: 10.7668/hbnxb.2016.04.016.

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