Expression Analysis of TaCDPK2 Gene in the Process of Wheat Infected by Puccinia triticina

doi:10.3969/j.issn.1000-7091.2012.02.004

Abstract

Abstract: The CDPK2-EST was isolated from the wheat SSH cDNA library induced by leaf rust，which has a?high level expression in 8h after inoculated leaf rust in incompatible combination． CDPKs (calcium-dependent protein kinases)are key elements in decoding the changes in cytosolic calcium concentrations during the plant defense?responses． The Western Blotting analysis and semi-QRT-PCR analysis were used to further investigate relative expression of the CDPK2 in different time points after wheat inoculated leaf rust． These results showed that the expression of CDPK2 was obviously increased in the incompatible combination compared with the control whether at the?levels of mRNA or protein at 4 h after inoculation，and the level of mRNA was declined to that in control level at 16?h which the protein level reached the maximum at the meantime． In compatible combination，TaCDPK2 was detectable at 8 h after inoculation，then declined to the normal level． These results implied that TaCDPK2 was involved in?leaf rust resistance in wheat，，and laid the foundation for further function research of TaCDPK2 in the further．

Key words: CDPKs, Puccinia triticina, Wheat

CLC Number:

CHENG Zi-yi, WEI Feng-ju, YAN Ai-hua, ZHANG Yun-wei, WANG Dong-mei. Expression Analysis of TaCDPK2 Gene in the Process of Wheat Infected by Puccinia triticina[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 19-22. doi: 10.3969/j.issn.1000-7091.2012.02.004.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3969/j.issn.1000-7091.2012.02.004

http://www.hbnxb.net/EN/Y2012/V27/I2/19

References

[1] 杨文香，田平素，黄燕，等．小麦叶锈菌离体培养研究[J]．河北农业大学学报，2001，24(4):58－61．
[2] Harmon A C，Gribskov M，Gubrium E，et al． The CDPKsuperfamily of protein kinases[J]． New Phytologist，2001，151:175－183．
[3] Hrabak E M，Chan C W M，Gribskov M，et al． The Arabidopsis CDPK-SnRK superfamily of protein kinases[J]．Plant Physiol，2003，132:666－680．
[4] Harper J F，Sussman M R，Schaller G E，et al． A calciumdependent protein kinase，with a regulatory domain similarto calmodulin[J]． Science，1991，252:951－954．
[5] Cheng S H，Willmann M R，Chen H C． Calcium signalingthrough protein kinases． The Arabidopsis calciumdependent protein kinase gene family[J]． Plant Physiol，2002，129:469－485．
[6] Reddy A S． Calcium:silver bullet in signalling[J]． PlantSci，2001，160:381－404．
[7] Sanders D，Pelloux J，Brownlee C，et al． Calcium at thecrossroads of signalling[J]． Plant Cell，2001，14:S401－S417．
[8] Zhang X Q，Chollet R． Seryl-phosphorylation of soybeannodule sucrose synthase(nodulin-100)by a Ca2 +-dependent protein kinase[J]． FEBS Lett，1997，410:126－130．
[9] Hernandez S C，Hardin S C，Clouse S D，et al． Identification of a new motif for CDPK． Phosphorylation in vitro thatsuggests ACC synthase may be a CDPK substrate[J]．Arch Biochem Biophys，2004，428:81－91．
[10] Botella J R，Arteca J M，Somodevilla M，et al． Calciumdependent protein kinase gene expression in response tophysical and chemical stimuli in mungbean(Vigna radiata)[J]． Plant Mol Biol，1996，30:1129－1137．
[11] Frattini M，Morello L，Breviario． Rice calcium-dependentprotein kinase isoforms OsCDPK2 and OsCDPK11 showdifferent responses to light and different expression patterns during seed development[J]． Plant Mol Biol，1999，41:753－764．
[12] Urao T，Katagiri T，Mizoguchi T，et al． Two genes thatencode Ca2 +-dependent protein kinases are induced bydrought and high-salt stresses in Arabidopsis thaliana[J]． Mol Gen Genet，1994，244:331－340．
[13] Saijo Y，Hata S，Kyozuka J，et al． Overexpression of asingle Ca2 +-dependent protein kinase confers both coldand salt/drought tolerance on rice plants[J]． Plant J，2000，23:319－327．
[14] Murillo I，Jaeck E，Cordero M J，et al． Transcriptional activation of a maize calcium-dependent protein kinasegene in response to fungal elicitors and infection[J]．Plant Mol Biol，2001，45:145－158．
[15] Wan B，Lin Y，Mou T． Expression of rice Ca2 +-dependent protein kinases(CDPKs)genes under different environmental stresses[J]． FEBS Lett，2007，581:1179－1189．
[16] Martinez-Noel G，Nagaraj V J，Calo G，et al． Sucroseregulated expression of a Ca2 +-dependent protein kinase(TaCDPK1)gene in excised leaves of wheat[J]． PlantPhysiol Biochem，2007，45:410－419．
[17] Zhu S Y，Yu X C，Wang X J，et al． Two calcium-dependent protein kinases，CPK4 and CPK11，regulate abscisic acid signal transduction in Arabidopsis[J]． PlantCell，2007，19:3019－3036．
[18] Mori I C，Murata Y，Yang Y，et al． CDPKs CPK6 andCPK3 function in ABA regulation of guard cell s-typeanion-and Ca2 +-permeable channels and stomatal closure[J]． PLOS Biol，2006，4:1749－1762．
[19] Li Ai-li，Zhu Yuan-fang，Tan Xiao-mei，et al． Evolutionary and functional study of the CDPK gene family inwheat(Triticum aestivum L．)[J]． Plant Mol Biol，2008，66:429－443．
[20] 关春蕾，侯春燕，王冬梅．影响 Ca2 +代谢和钙通道的药物对小麦受叶锈菌侵染后诱发的 HR 的作用[J]．河北农业大学学报，2006，29(6):4－8．
[21] 张蓓，阎爱华，刘刚，等．胞内钙库对小麦叶锈菌侵染之过敏反应的影响[J]．作物学报，2010，36(5):833－839．
[22] 侯春燕，王智炘，王冬梅．钙与叶锈菌侵染诱导小麦防卫反应的关系[J]．华北农学报，2007，22(1):145－147．
[23] 白志英，王冬梅，侯春燕，等．小麦与叶锈菌互作过程中细胞程序性死亡的生化证据简报[J]．实验生物学报，2004，37(4):329－332．
[24] Yan A H，Zhang L F，Zhang Y W，et al． Early stage SSHlibrary construction of wheat near-isogenic line TcLr19under the stress of Puccinia recondita f． sp． Tritici[J]．Frontiers of Agriculture in China，2009，3 (2):146－151．
[25] 朱广联．植物生理生化实验[M]．北京:高等教育出版社，1983:240－244．

Please choose a citation manager

Content to export