Cloning and Expression of a Potato Dirigent-like Gene(StDIR1) Responsive to Infection by Phytophthora infestans

doi:10.3969/j.issn.1000-7091.2012.02.005

Abstract

Abstract: Potato is an important vegetable and food crop all over the world． Potato late blight，caused by Phytophthora infestans，is one of the most serious challenges in potato breeding in which an essential method is to isolate?the resistance genes and gain the new resistant potato varieties by transgenic technology． Dirigent protein，found in?Forsythia suspense firstly，assists the stereoselective bimolecular phenoxy radical coupling in lignan synthesis，and?may play an important role in plant resistance against pests and pathogens． Specific primers were designed according?to the potato EST sequence，and a 729 bp cDNA，designated StDIR1，was cloned by RT-PCR and RACE amplification，which encodes a peptide composed of 191 amino acids and contains a conservative domain of dirigent proteins．?Phylogenetic analysis indicated that the assumption peptide，which shares a similarity of 76% and an identity of?62% with Gossypium hirsutum Dirigent-like protein 1，is a Dirigent-like protein and belongs to the DIR-b subfamily．?Semi-quantitative RT-PCR analysis indicated that the expression of StDIR1 is up-regulated in different degrees under the induction of P． infestans，exogenous H2O2and NO，respectively． Tissue-specific expression analysis demonstrated a very high expression level of StDIR1 in potato root tissue，and a lower level of expression in the tissue of?stem，leaf，flower and tuber． This study firstly indicated that the StDIR1 gene is related to the response of potato against the infection of P． infestans．

Key words: Dirigent protein, Potato late blight, Plant disease resistance

CLC Number:

S432.1
Q78

ZHANG Hong-wei, LI Ji-gang, ZHENG Jian-po, QU Zhan-liang. Cloning and Expression of a Potato Dirigent-like Gene(StDIR1) Responsive to Infection by Phytophthora infestans[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 23-29. doi: 10.3969/j.issn.1000-7091.2012.02.005.

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