Cloning and Expression Analysis of ZmCBL2-2 Gene from Maize (Zea mays． L)

doi:10.3969/j.issn.1000-7091.2012.02.006

Abstract

Abstract: To analyze the role of ZmCBL2-2 gene in the tolerance of maize to abiotic stresses by methods such?as transgenic plants，the complete coding region cDNA fragment of the gene was cloned by reverse transcription polymerase chain reaction (RT-PCR) and constructed into the plant expression vector p3301 (pCAMBIA3301)． Moreover，the expression profiles of ZmCBL2-2 under six abiotic stresses such as high salinity，low temperature and nutritional deficiency were analyzed by real time quantitative PCR (RT-qPCR) method，and it was found that the gene?responded significantly to nitrogen deprivation，low potassium，low temperature，exogenous abscisic acid (ABA) and?water stress，but did not respond obviously to salt stress．

Key words: Maize, CBL, Gene cloning, Expression analysis

CLC Number:

S513
Q78

LI Ji-yuan, WANG Cun-fang, ZHAO Huan-huan, WANG Mao-yan. Cloning and Expression Analysis of ZmCBL2-2 Gene from Maize (Zea mays． L)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 30-34. doi: 10.3969/j.issn.1000-7091.2012.02.006.

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http://www.hbnxb.net/EN/Y2012/V27/I2/30

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