Effects of Different Rootstocks on Huangguogan Fruit Organic Acid Content, Acid Metabolism-related Enzyme Activity and Gene Expression

doi:10.7668/hbnxb.2016.04.014

Abstract

Abstract: In order to explore the effects of different rootstocks on fruit organic acid,acid metabolism-related enzyme activities and the gene expression,to provide theoretical evidence for the selection of the rootstocks.In this experiment with Poncirustrifoliate (L.)Raf,Citrus reticulate Blanco,C.junos (sieb.) Tanaka for the rootstocks from Huangguogan seeding as the control for study.Showed that:Huangguogan fruit had been accumulated mainly citric acid,C.junos (sieb.) Tanaka could most effectively reduce the organic content of the fruit,mature period was 24.35% lower than in the the seedling.Grafting can be reduced to some extent the activity of CS,but has little effect on the activity of PEPC and MDH.250-330 days after flowering,the ACO and NADP-IDH activity exhibited a trend of C.junos (sieb.) Tanaka > Poncirustrifoliate (L.)Raf > Citrus reticulate Blanco > CK.In addition to seeding,Huangguogan fruit between the level of expression of NADP-IDH and total acid content was significantly negatively correlated,there was no significant correlation between CS,MDH,ACO and total acid content.The C.junos(sieb.) Tanaka is ideal rootstock varieties of Huangguogan,Organic acid content of different rootstocks fruit is the result of combined action of the ACO and NADP-IDH activity,and NADP-IDH gene expression may be the mainly limiting factor of Huangguogan fruit organic acid with different rootstocks.

Key words: Huangguogan, Rootstock, Organic acid, Enzyme activity, Gene expression

CLC Number:

S666.9
Q78

CAO Shuyan, RONG Yi, GU Xianjie, LI Qingnan, LIAO Ling, YE Shuang, QIU Xia, WANG Zhihui. Effects of Different Rootstocks on Huangguogan Fruit Organic Acid Content, Acid Metabolism-related Enzyme Activity and Gene Expression[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(4): 80-87. doi: 10.7668/hbnxb.2016.04.014.

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