An EMS-induced Mutant with 2S Protein Deficiency in Seed Storage Proteins of Camelina sativa

doi:10.7668/hbnxb.2016.03.002

Abstract

Abstract: Camelina sativa is an important oilseed crop, whose seeds is full of high content of unsaturated fatty acids and contain lots of storage proteins.In order to obtain extensive breeding material and establish superior new germplasm,camelina seeds were treated by EMS (ethyl methane sulphonate).In this study, Camelina sativa seeds were treated for mutation by EMS (ethyl methane sulphonate).The treatments were conducted with different EMS concentrations (0,0.2%,0.4%,0.6%,0.8%,1.0%,1.2%) and various treating times (0,8,12,16 h).An optimizational mutagenesis system by EMS was developed for Camelina sativa seeds.One mutant showing 2S albumin deficiency in seed proteins was obtained,and designated as 2SP1 .Except for this feature,the mutant exhibited no significant difference from the wild-type camelina for other agronomic traits including seed oil and protein contents,plant morphology and seed germination.This special mutant of 2SP1 could be used as a new excellent germplasm for investigating the mechanism of seed storage protein biosynthesis and accumulation,for breeding new Camelina sativa varieties with high nutrition quanlity,and also for further functional genomics research.

Key words: Camelina sativa, 2S albumin/napin, EMS, Mutant (2SP1)

CLC Number:

YUAN Lixia, HAO Jingyun, ZHAO Kui, XUE Jinai, LI Runzhi. An EMS-induced Mutant with 2S Protein Deficiency in Seed Storage Proteins of Camelina sativa[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 11-17. doi: 10.7668/hbnxb.2016.03.002.

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