Response of Enzyme Activities and Growth Vigoron of Walnut to Jugladis Canker Stress

doi:10.7668/hbnxb.201751454

Abstract

Abstract: To explore the interaction between the biochemical metabolism of walnut branches and the disease resistance of trees under the stress of Jugladis canker.Randomly selected from health, mild, moderate and severe degree of 4 kinds of disease plant each 7 plants, then use Duncan (P<0.05) and single factor analysis of variance blade 3 branches in 3 different antioxidant enzyme activity, multivariate linear regression analysis by using the PAL (phenylalanine ammonia-lyase) activity, POD (peroxidase) activity and Pro (proline) of branches,and walnut base stem relates to the degree of disease (disease spot size).Results showed that the change of the three kinds of antioxidant enzyme activity had certain difference, the PAL activity peak appears in the mild disease leaves and moderate disease branches, while the POD activity of maximum health plant leaves and branches moderate disease, leaf Pro and POD activity of showed similar rule, samples were significantly higher than other disease activity (P<0.05).In addition, the activity of branch Pro had a bimodal characteristic, that is, its activity increased rapidly at the initial stage of infection, while its activity decreased significantly if the disease was further aggravated, and increased significantly with the development of disease.The minima PAL and Pro activity appeared in the early stage of disease infection, and POD activity minima appeared in the late stage of disease development.Correlation analysis showed that POD activity and walnut growth (walnut base stem) had significant negative effects on disease degree, and the correlation reached -0.76 (P<0.01) and -0.85 (P<0.01), respectively.The disease degree was significantly affected by Pro, and the correlation was 0.52 (P<0.01).Therefore, during the infection process of rot disease, increasing the growth of walnut, that is, increasing the base and stem of walnut, has a strong inhibitory effect on the infection process of rot disease. In addition, it also showed that the increased POD activity in walnut plants is conducive to strengthening the resistance of walnut to rot disease.

Key words: Jugladis canker, POD activity, Proline activity, Stems of walnut, Canker area, Multiple linear regression analysis

CLC Number:

S664.1

FENG Lei, GENG Zengchao, XU Wanli, XUE Quanhong, SUN Ningchuan, GU Yuzhong, TANG Guangmu. Response of Enzyme Activities and Growth Vigoron of Walnut to Jugladis Canker Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(4): 217-222. doi: 10.7668/hbnxb.201751454.

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References

[1] 张天勇. 核桃腐烂病发生规律及防治技术[J]. 陕西林业科技,2012(3):78-79,82.doi:10.3969/j.issn.1001-2117.2012.03.024.
Zang T Y. Techniques to control and prevent rot in walnut[J]. Shaanxi Forest Science and Technology,2012(3):78-79,82.
[2] 张志华,刘新彩,王红霞,高仪,赵悦平. 核桃IOD和POD酶活性与生长势的关系[J]. 园艺学报,2006,33(2):229-232.doi:10.3321/j.issn:0513-353X.2006.02.002.
Zang Z H,Liu X C,Wang H X,Gao Y,Zhao Y P. Studies on the relationship between the POD and IOD activity and the growth vigor of walnut[J]. Acta Horticulturae Sinica,2006,33(2):229-232.
[3] 周斌,张金龙,冯纪年,王敦. 短期低温胁迫对核桃抗氧化酶活性的影响[J]. 西北林学院学报,2015,30(3):51-53,65. doi:10.3969/j.issn.1001-7461.2015.03.09.
Zou B,Zhang J L,Feng J N,Wang D. Antioxidant enzymes activities of walnut tree under short term cold stress[J]. Journal of Northwest Forestry University,2015,30(3):51-53,65.
[4] 相昆,张美勇,徐颖,王晓芳,岳林旭. 不同核桃品种耐寒特性综合评价[J]. 应用生态学报,2011,22(9):2325-2330. doi:10.13287/j.1001-9332.2011.0328.
Xang K,Zhang M Y,Xu Y,Wang X F,Yue L X.Cold-tolerance of walnut cultivars:A comprehensive evaluation[J]. Chinese Journal of Applied Ecology,2011,22(9):2325-2330.
[5] Biggs A R,Miles N W. Association of suberin formation in uninoculated wounds with susceptibility to Leucostoma cincta and L. persoonii in various peach cultivars[J]. Phytopathology,1988,78(8):1070-1074. doi:10.1094/Phyto-78-1070.
[6] 孙广宇,卫小勇,孙悦,肖云学,商圣平,朱明旗,郭云忠.苹果树腐烂病发生与叶片营养成分的关系[J]. 西北农林科技大学学报(自然科学版),2014,42(7):107-112,121. doi:10.13207/j.cnki.jnwafu.2014.07.008.
Sn G Y,Wei X Y,Sun Y,Xiao Y X,Shang S P,Zhu M Q,Gho Y Z. Relationship between occurrence of apple Valsa canker and leaf nutrients[J]. Journal of Northwest A&F University(Natural Science Edition),2014,42(7):107-112,121.
[7] 袁朝兴,丁静. 水分胁迫对棉花叶片中IAA从含量、IAA氧化酶和过氧化物酶活性的影响[J]. 植物生理学报,1990,16(2):179-180.
Yan C X,Ding J. Effects of water stress on the content of IAA and the activities of IAA oxidase and peroxidase in cotton leaves[J]. ACTA Phytophysiologia Sinica,1990,16(2):179-180.
[8] 张殿忠,汪沛洪,席连喜.干物质累积和脯氨酸累积的水势阈值与小麦抗旱性的关系[J]. 干旱地区农业研究,1990(2):66-71.
Zang D Z,Wang P H,Xi L X. Relationship between dry matter accmulication and water potential threshold of free proline accmulication and wheat resistance to drought[J]. Agricultural Research in the Arid Areas,1990(2):66-71.
[9] 王敬文,薛应龙. 植物苯丙氨酸解氨酶的研究Ⅰ.植物激素对甘薯块根苯丙氨酸解氨酶和肉桂酸4-羟化酶活性变化及其伴随性的影响[J]. 植物生理学报,1981,7(4):373-380.
Wng J W,Xue Y L. Studies on plant phenylalanine ammonia-lyase Ⅰ.the effect of phytohormone on the increase in phenylalanine ammonia-lyase(PAL)and cinnamic acid 4-hydroxylase(CA4H)activity and the sequence of concomitant changes of enzyme activities in sweet potato root tuber discs[J]. ACTA Phytophysiologia Sinica,1981,7(4):373-380.
[10] Koukol J,Conn E E. The metabolism of aromatic compounds in higher plants. IV. Purification and properties of the phenylalanine deaminase of Hordeum vulgare[J]. Journal of Biological Chemistry,1961,236(10):2692-2698.
[11] 徐建华,利容千,王建波. 黄瓜不同抗病品种感染镰刀菌枯萎病菌后几种酶活性的变化[J]. 植物病理学报,1995,25(3):239-242. doi:10.1007/BF02007173.
X J H,Li R Q,Wang J B. Some changes of enzyme activities from susceptible and resistance cucumber cultivars after inoculation with cucumber Fusarium wilt[J]. Acta Phytopathologica Sinica,1995,25(3):239-242.
[12] 刘佳. 美洲南瓜苯丙氨酸解氨酶(PAL)基因克隆、表达分析及品种抗灰霉病研究[D].兰州:甘肃农业大学,2013.
Lu J. Cloning and expression analysis of phenylalanine ammonia-lyase(PAL)gene in American pumpkin and study on its resistance to grey mould[D].Lanzhou:Gansu Agricultural University,2013.
[13] 马静芳,李敏权,张自和,柴兆祥. 苯丙氨酸解氨酶与苜蓿种质根和根颈腐烂病抗病性研究[J]. 草业学报,2003,12(4):35-39. doi:10.3321/j.issn:1004-5759.2003.04.007.
M J F,Li M Q,Zhang Z H,Chai Z X. Study on relationship between phenylalanine ammonia-lyase(PAL)and resistance to crown and root rot in alfalfa cultivars[J]. Acta Prataculturae Sinica,2003,12(4):35-39.
[14] 程鹏,徐鹏飞,范素杰,靳立梅,吴俊江,王金生,张淑珍. 野生大豆接种大豆疫霉根腐病菌后过氧化物酶(POD)活性变化[J]. 大豆科学,2013,32(2):197-201. doi:10.3969/j.issn.1000-9841.2013.02.013.
Ceng P,Xu P F,Fan S J,Jin L M,Wu J J,Wang J S,Zhang S Z. Response of POD activity in glycine soja inoculated by phytophthora sojae[J]. Soybean Science,2013,32(2):197-201.
[15] 陈臻,侯宝宏,王卫雄,徐秉良. 黄腐酸处理对苹果树腐烂病菌的抑制作用及对苹果树防御酶活性的影响[J]. 植物保护,2016,42(3):81-86,103. doi:10.3969/j.issn.0529-1542.2016.03.013.
Cen Z,Hou B H,Wang W X,Xu B L. Inhibition of fulvic acid to Valsa mali and its effect on the activity of defense enzyme in apple tree[J]. Plant Protection,2016,42(3):81-86,103.
[16] 刘普,施圆圆,叶振风,衡伟,贾兵,刘莉,张水明,朱立武. 梨树腐烂病研究进展[J]. 安徽农业大学学报,2014,41(4):695-700.doi:10.13610/j.cnki.1672-352x.20140620.027.
Lu P,Shi Y Y,Ye Z F,He W,Jia B,Liu L,Zhang S M,Zhu L W. Research progress in pear Valsa canker[J]. Journal of Anhui Agricultural University,2014,41(4):695-700.
[17] 张新慧,张恩和,王惠珍,郎多勇. 连作对当归生长的障碍效应及机制研究[J]. 中国中药杂志,2010,35(10):1231-1234.doi:10.4268/cjcmm20101003.
Zang X H,Zhang E H,Wang H Z,Lang D Y. Effects of continuous cropping obstacle on growth of Angelica sinensis and its mechanism[J]. China Journal of Chinese Materiamedica,2010,35(10):1231-1234.
[18] 湛蔚,刘洪光,唐明. 菌根真菌提高杨树抗溃疡病生理生化机制的研究[J]. 西北植物学报,2010,30(12):2437-2443.
Zan W,Liu H G,Tang M. Physiological and biochemical mechanism of mycorrhizal fungi improving the resistance of poplar to canker disease[J]. Acta Botanica Boreali-occidentalia Sinica,2010,30(12):2437-2443.
[19] 吴玉霞,何天明,李疆,刘博,马少兴,周梦新,李芳芳. 高位嫁接提高库尔勒香梨越冬性的初步研究[J].西北农业学报,2012,21(6):129-133.doi:10.7606/j.issn.1004-1389.2012.6.025.
W Y X,He T M,Li J,Liu B,Ma S X,Zhou M X,Li F F. Increasing cold hardiness of kuerle fragrant pear(Pyrus bretschneideri) by Top-grafting[J]. Acta Agriculturae Boreali-occidentalis Sinica,2012,21(6):129-133.
[20] 樊祥伦. 果树除萌和摘心减轻腐烂病发生[J].北方园艺,1990(8):24.
Fn X L. Fruit tree desprout and heart pick reduce the occurrence of putrefaction[J]. Northern Horticulture,1990(8):24.
[21] 肖云学. 苹果树腐烂病发生影响因素及防治研究[D].杨凌:西北农林科技大学,2013.
Xao Y X. Study on the influencing factors and prevention of apple tree rot[D].Yangling:Northwest A&F University,2013.
[22] 冯雷,徐万里,薛权宏,唐光木,孙宁川,顾玉忠. 矿质元素对核桃腐烂病病害程度的影响[J].经济林研究,2017,35(4):49-56. doi:10.14067/j.cnki.1003-8981.2017.04.008.
Fng L,Xu W L,Xue Q H,Tang G M,Sun N C,Gu Y Z.Effects of mineral elements on harm degree of Juglans regia canker[J].Nonwood Forest Research,2017,35(4):49-56.
[23] 李美美,马华冰,宋新英,齐国辉,李保国. 不同修剪措施对成龄‘绿岭’核桃抗腐烂病能力及树势的影响[J]. 经济林研究,2014(3):61-67.doi:10.3969/j.issn.1003-8981.2014.03.010.
L M M,Ma H B,Song X Y,Qi G H,Li B G. Effects of different pruning methods on rot disease resistant and tree vigor of adult ‘Lvling’ walnut[J]. Nonwood Forest Research,2014(3):61-67.

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