EMS Mutagenesis Analysis of Foxtail Millet Shilixiang

doi:10.7668/hbnxb.2017.S1.019

Abstract

Abstract: The optimal mutagenic conditions of foxtail millet ripening seeds under different concentrations of EMS mutagenesis were discussed, which laid material foundations for screening rust-sensitive mutant of Shilixiang to carry out basic research.The rust-resistant variety of foxtail millet,Shilixiang,as this study materials.Both of the seeds with soaking in advance and without soaking were treated with different concentrations of EMS(0,0.8%, 1.0%,1.2% and 1.5%) for 16 hours,then the germination potential,germination rate and germination length were measured at the 3 rd and 7 th day after treatment.The germination potential and germination rate of Shilixiang seeds decreased with the increase of EMS concentration.When the EMS concentration of 1.2% and 1.5%, the seed germination rate is not affected by the soaking in advance or not. In addition, under the same EMS concentration treatment,both the germination potential and the germination rate of Shilixiang seeds with soaking in advance were higher than those without soaking.The germination potential was the most significant (9.66%) at EMS concentration of 1.2%,followed by 1.0%. And the germination rate was the most significant (8.66%) at EMS concentration of 1.0%,but no difference at EMS concentration of 1.2%and 1.5%. The higher the EMS concentration was,the faster the germination potential and germination rate of the Shilixiang seeds decreased. As the EMS concentration increased from 1.2%to 1.5%,both the germination potential and the germination rate decreased by 36.34%-40.33% and 44.00%-48.00%,respectively. When the EMS concentration was 1.5%,they reached the lowest level. With the increased of the EMS concentration,the germination length of Shilixiang seeds became shorter,and the change of shoot length was the most significant at EMS concentration of 1.5%.EMS could significantly inhibit the seed germination,and the greater the concentration was,the more obvious the inhibitory effect was. With the seed germination rate of 50.00%as the standard,the optimum concentration of EMS was 1.2%in the condition of pre-soaking or not soaking.

Key words: Foxtail millet, EMS, Mutagenesis, Germination potential, Germination rate

CLC Number:

S515.03

SONG Zhenjun, LI Zhiyong, QUAN Jianzhang, SHI Can, LIU Lei, MA Jifang, WANG Yongfang, BAI Hui. EMS Mutagenesis Analysis of Foxtail Millet Shilixiang[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(S1): 108-113. doi: 10.7668/hbnxb.2017.S1.019.

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