SDI PAGE Analysis of Phase Change- related Proteins in Phloem in Apple( Malus domestics Borhk.)

doi:10.7668/hbnxb.2009.01.025

Abstract

Abstract: Dyanic changes of proteins in phloem from different nodes in 5 hybrid seedlings( Jonathan x Golden De-licious)were analyzed with SDI polyac amide gel electrophoresis. The result indicated that protein bands changed withnodes increasing.Three prater bands found in different phases. The molecular weight of the 3 protein bands were17. 0, 21. 0, 55. 0 kDa respectivly.The 55. 0 kDa protein presented qualitative change which appeared in the node No.46 and disappeared the node No. 125. Changes of 17. 0 kDa and 21. 0 kDa proteins were found quantitaively. In phloemsamples taken below nodes No. 111-125, the content of 21. 0 kDa protein was higher, but a sharp decrease occurred inphloem above nodes No. 125. The content of 17. 0 kDa protein kept high bet nodes No. 51-65 and No. 106- 120.

Key words: Malus domestacrz, Phase change, Protein, SDI-PAGE

CLC Number:

S661.1

TENG Yun-long, LI Chun-min, ZHAO Yong-bo, ZENG Guang-juan, CHEN Dong-me, DONG Wen-xuan, ZHANG Xin-zhong. SDI PAGE Analysis of Phase Change- related Proteins in Phloem in Apple( Malus domestics Borhk.)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 112-114. doi: 10.7668/hbnxb.2009.01.025.

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