Detection of High Effect Site for Resistance to Rice Stripe Virus by BSA

doi:10.7668/hbnxb.2014.02.016

Abstract

Abstract: The Jiangsu landrace rice variety Handao was previously identified as high resistance to rice stripe disease (RSV) . A total of 141 F_2:3 lines derived from the cross of Handao and Wuyujing 3, a variety high sensitive to RSV, were investigated by natural infection method in field, and the disease rating index was scored. Analysis of the resistant/sensitive pools by bulked segregant analysis indicated that the SSR markers RM209 and RM21 on rice chromosome 11 obviously linked to RSV resistance. A QTL for RSV was detected on rice chromosome 11 between RM209 and RM21 by the software QTL IciMapping V3. 2 based on the principle of inclusive composite interval mapping (ICIM) with the maximum LOD score 13. 25, which explained 38. 39% of the trait variance. Additive effects of qSTV11^HD was negative, indicating that the resistance allele was inherited from Handao.

Key words: Rice, Rice stripe virus, Bulked segregant analysis, QTL mapping

CLC Number:

S435.111.4+2

ZHANG Yun-hui, ZHANG Suo-bing, LIN Jing, WANG Ying-jie, FANG Xian-wen. Detection of High Effect Site for Resistance to Rice Stripe Virus by BSA[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(2): 85-88. doi: 10.7668/hbnxb.2014.02.016.

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