Establishment and Optimization of Flipper Transposon PCR Amplification System of the Grape Botrytis cinerea

doi:10.3969/j.issn.1000-7091.2013.03.041

Abstract

Abstract: Grape gray mold caused by Botrytic cinerea Pers． is one of the most important diseases on grapes worldwide． The genetic variation of Botrytis cinerea is widely and adaptable，which is easy to produce drug-resistant strains． It is reported that Boty and Flipper transposon of Botrytis cinerea are related to the presence or absence of resistant strains． By setting different gradient and optimize Botrytis cinerea Flipper transposon PCR amplification system of grape in China， including template DNA，primer，dNTPs， 10 × Taq 10 Buffer，Taq DNA polymerase，annealing temperature，cycle indexthe 1 159 bp fragment was obtained ，which has 99% similarity with Botryotinia fuckeliana Flipper transposable element transposase gene through NCBI blastn． So the amplified fragment is verified as expected target fragment． The establishment and optimization of Flipper transposon PCR amplification system of the grape Botrytis cinerea in China provide an important foundation to further study of the relationship between the transposable elements，pathogenicity and resistant

Key words: Botrytis cinerea, Flipper transposable elements, PCR amplification system, Single factor secreening

CLC Number:

Q78
S436.6

Chang Jiaying, Kou Hongda, Qiao Danna, Zhang Yanjie, Chi Guotong, Li Yaning, Li Xinghong, Liu Daqun. Establishment and Optimization of Flipper Transposon PCR Amplification System of the Grape Botrytis cinerea[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 227-233. doi: 10.3969/j.issn.1000-7091.2013.03.041.

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