Detection of Corynespora cassiicola in Soil with Real-time Quantitative PCR

doi:10.7668/hbnxb.2014.02.013

Abstract

Abstract: In this research, a specific primer pair CIR5 /CIF5 for C. cassiicola was developed, and Real-time PCR based specific detection method of this pathogen in soil was established. The primer pair gave a single amplifi- cation of 259 bp from C. cassiicola and could be distinguished from other soil-borne pathogen strains. The detection limit was 10 fg/μL in conventional PCR. The pathogen in soil could be detected by Real-time quantitative PCR, and the sensitivity was down to C. cassiicola DNA of 1 conidia/g. This Real-time quantitative PCR method could assist in the implementation of quarantine measures for prevention and control of Corynespora spot leaf.

Key words: Corynespora cassiicola, Real-time quantitative PCR, Soil detection

CLC Number:

S642.2

GAO Wei, LI Bao-ju, WANG Wan-li, HAO Yong-juan, SHI Yan-xia. Detection of Corynespora cassiicola in Soil with Real-time Quantitative PCR[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(2): 71-74. doi: 10.7668/hbnxb.2014.02.013.

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