Abstract: In order to analyze the differentiation of different areas Corynespora cassiicola and identify the pathogen accurately so as to serve the cucumber disease resistance breeding,34 corynespora cassiicola purified strains was collected from 2007 to 2014.Molecular identification results showed that all the isolates were Corynespora cassiicola using published specific primers CCF/CCR,all the 34 strains were amplified the expected 272 bp specific fragments.559 bp fragments of ribosomal DNA internal transcribed spacer sequences of corynespora cassiicola were obtained through PCR amplification by using universal primers ITS1 and ITS4.Sequence analysis result showed that there was no difference among the sequences of ribosomal DNA ITS of 32 strains isolated from cucumber,only 2 nucleotides mutation comparing with the sequence of the strains isolated from tomato,the similarity was 99.64%.The above results showed that the ITS sequences were highly conserved in Corynespora Güssow,the ITS sequence might be used for differential identification of species of Corynespora,the ITS sequence can distinguish different isolates of Corynespora cassiicola fromcucumber and tomato host.

Key words: Cucumber, Corynespora cassiicola (Berk.&Curt.)Wei, Molecular identification, rDNA-ITS

CLC Number: 

• S642.2

• Q78