Abstract: In order to make a foundation for illustrating the pathogenic mechanism of G protein beta-subunit in Curvularia lunata,this research focuses on acquiring the gene encoding Gβ subunit in C.lunata and exploring its expression pattern.The full-length cDNA of G-protein beta-subunit was cloned using SMART RACE RT-PCR,and the expression pattern of ClGβ gene was analyzed under different growth time using Real-time PCR.The full-length cDNA of ClGβ was 1 056 bp and encoded 351 amino acids,while its DNA contained 4 introns and 5 extrons.The cDNA sequence of ClGβ had been deposited in GenBank with accession number JQ768316.The results of Real-time PCR indicated that the gene expression level of G-protein beta-subunit is lower at early stage and higher at later stage.We constructed the prokaryotic expression vector pET28(a)-ClGβ,and E.coli BL21 was transformed by this recombinant construct and induced by IPTG.The molecular weight of the expression product was identical with the calculated molecular weight of ClGβ.

Key words: Curvularia lunata, G protein beta-subunit, Real-time quantitative PCR, Prokaryotic expression

CLC Number: 

• Q78