Involvement of Small GTP-Binding Protein Gene Rab2 in Resistance Response of Wheat to Its Leaf Rust Disease

doi:10.7668/hbnxb.2010.04.001

Abstract

Abstract: Wheat leaf rust disease caused by wheat leaf rust (Puccinia triticina) is one of the most important diseases which have led to heavy losses to the wheat production in the world，and it also is a main factor influencing the wheat production in our country. The application of the disease resistant varieties and increasing host plant resistance are the most promising ways to control this disease，which based on the fully understanding of the resistant mechanisms. In recent years，the regulation channel of small G protein has become a hot issue of research of plant resistant mechanism. The small GTP-binding protein Rab，which consists of about two hundred amino acids，is the largest one of small GTP-binding proteins sub-families and plays an very important role in vesicular transport in eukaryotic cells. The purpose of this study is to understand the expression of the small G protein Rab2 gene in the wheat infected with wheat leaf rust (Puccinia triticina) ，and definite the relationship between the Rab2 and the disease resistance，to investigate the resistance mechanism of wheat leaf rust in the molecular level. In this study，the near-isogenic lines of the common hexaploid wheat TcLr1 and leaf rust Race 05-22-64 ①and 05-8-63① are used to build the compatible combinations and the incompatible combinations，then RNA were extracted at different time points and the first strand of cDNA were synthesized by reverse transcription in vitro. Rab2 gene expression were analyzed by real-time fluorescence quantitative PCR. As results，in the process of infection by wheat leaf rust (puccinia triticina) ，Rab2 gene significantly expressed only in six hours after inoculation and reached the highest expression point at twelve hours after inoculation， a nd in the following hours Rab2 gene expression decreased. It means Rab2 gene is mainly expressed in the early stage of infection，while declines with time prolonged. The incompatible combinations can upregulate the expression of the small GTP-binding protein Rab2 gene，while the compatible combinations will depress its expression. Therefore，the small G protein should be directly related to the affinity of the host and its pathogen.

Key words: Wheat leaf rust disease, Small G protein, Rab2 gene, Real-time quantitative PCR

CLC Number:

YANG Jing-jing, LI Xing, LI Ya-ning, LIU Da-qun. Involvement of Small GTP-Binding Protein Gene Rab2 in Resistance Response of Wheat to Its Leaf Rust Disease[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 1-5. doi: 10.7668/hbnxb.2010.04.001.

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