Cloning of MdCaM and Its Expression Responses under Abiotic Stresses During Malus domestica Postharvest Storage

doi:10.7668/hbnxb.2017.01.008

Abstract

Abstract: In order to identify the function of MdCaM gene in apple under different abiotic stresses. MdCaM gene was cloned by RT-PCR from apple, and phylogenetic tree was clustered based on amino acid of it. Also, the relative expression levels of MdCaM gene was analyzed by Real-time quantitative PCR (qPCR) method under wound, low-oxygen, high-temperature stress during apple fruit storage. The results showed that Calmodulin gene had high homology in different species, and MdCaM gene had a closer relationship with Arabidopsis thaliana CaM₁. qPCR analysis showed that the transcription level of MdCaM was up-regulated in the early stage, then returned to the normal level after wound and low-oxygen (0 O₂ and 3%O₂) stress, the difference was wound stress peak at 6 h, while low-oxygen (0 O₂) stress peak at 1 h. Also, MdCaM was up-regulated under high-temperature (20℃ and 40℃) stress compared to control,and the expression level was the highest at 12 h under stress,which indicated that MdCaM gene might be involved in the whole process of high-temperature stress-responsive. These results suggested that MdCaM might function as an stress-responsive gene under wound,high-temperature,low-oxygen stresses during apple storage. This research provided a theoretical basis for further study of the function and molecular regulation mechanism of MdCaM.

Key words: Apple, Postharvest stress, Real-time quantitative PCR, MdCaM, Gene clone

CLC Number:

Q78:S661.03

SU Liyan, TIAN Aimei, TAO Guirong, LI Meng. Cloning of MdCaM and Its Expression Responses under Abiotic Stresses During Malus domestica Postharvest Storage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(1): 47-52. doi: 10.7668/hbnxb.2017.01.008.

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