Primary Study on LBD Genes Involving in Callus Formation and Plant Regeneration of Chinese Cabbage

doi:10.7668/hbnxb.2015.03.016

Abstract

Abstract: Callus formation,embryogenesis and adventitious bud development are major steps of plant regeneration,in which callus formation is a key step.Previous reports demonstrated that exogenous auxin and LBD (Lateral organ boundaries domain) genes, LBD16,LBD17,LBD18 and LBD29,were involved in callus formation in Arabidopsis.Shoot regeneration frequency of 37 high inbred line of Chinese cabbage were analyzed and 2 of them,with significant different regeneration frequency,were selected for further study.Here,we focused on the function of Brassica LBD genes in callus formation and plant regeneration by analyzing LBD expression level during callus induction of Chinese cabbage with different regeneration capacity.The main results are as follows:BrLBD genes are highly homologous to Arabidopsis LBD genes,and a total of 8 relevant LBD homologous are found in Brassica genome.Visible callus tissue began to appear within 7 days after explant inoculation,and interestingly,Brassica LBD genes were detected to reach its highest expression level at the time point and the levels first increased and then decrease in the process of regeneration;At every time point during callus induction,LBD genes expressed a higher level in materials with high regeneration frequency than that with low regeneration frequency.All these results revealed that LBD genes were involved in callus formation and promoted plant regeneration in vitro.This may provide us a new perspective for large-scale screening of high-frequency regeneration materials to establish efficient genetic transformation system at the molecular level.

Key words: Brassica rapa ssp. pekinensis, Callus formation, Lateral organ boundaries domain, Real-time quantitative PCR

CLC Number:

S643.03

LI Qiang, SU Tong-bing, YU Shuan-cang, ZHANG Feng-lan, YU Yang-jun, ZHANG De-shuang, ZHAO Xiu-yun, WANG Wei-hong, LU Gui-xiang, ZHU Yue-lin. Primary Study on LBD Genes Involving in Callus Formation and Plant Regeneration of Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(3): 83-89. doi: 10.7668/hbnxb.2015.03.016.

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