Genetic Diversity Analysis of Waxy Corn Inbred Lines by Single Nucleotide Polymorphism (SNP) Markers

doi:10.7668/hbnxb.2015.03.015

Abstract

Abstract: In order to explore genetic diversity of waxy corn varieties,a novel molecular marker technology-Single nucleotide polymorphism (SNP) markers were adopted in genotypic analysis of 39 elite waxy corn inbred lines.Polymorphic information content (PIC) of 1 059 SNP markers on these inbred lines were 0.05-0.38,with average value being 0.31;Minor allele frequency (MAF) were 0.03-0.50,with average value being 0.29;Expected heterozygosity were 0.05-0.50,with an average of 0.39.Kinship coefficient between 39 inbred lines ranged from 0.00 to 0.91.Jing 6 and Jingnuo 6 had the strongest similarity.Five groups were divided from the 39 lines by Neighbor-joining (NJ) clustering method.Inbred lines with unclear pedigree were divided into different groups,so their relatedness with other lines were defined.Therefore,SNP markers were applicable to the analysis of genetic diversity and genetic relationships of waxy corn inbred lines,could provide data for utilization and developing new varieties in breeding program.

Key words: Waxy corn, SNP marker, Genetic diversity, Jingnuo 6, Bainuo 6

CLC Number:

S513.03

SHI Ya-xing, LU Bai-shan, SONG Wei, XU Li, ZHAO Jiu-ran. Genetic Diversity Analysis of Waxy Corn Inbred Lines by Single Nucleotide Polymorphism (SNP) Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(3): 77-82. doi: 10.7668/hbnxb.2015.03.015.

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