Cloning and Salt Stress Response Analysis of ZmRab7 Gene in Zea mays L.

doi:10.7668/hbnxb.20190741

Abstract

Abstract: To study the function of maize small G protein in salt stress, the ZmRab7 was identified and its role was characterized in tolerance to salt stress. Multiple sequence alignment showed that ZmRab7 encoded 206 amino acids, including conserved G1-G5 motifs and C-terminal Cys site. Phylogenetic analysis indicated that the amino acid sequence of ZmRab7 was highly homologous to SbRab7 in Sorghum bicolor. There were four α-helixes, several β-sheets and random coils in the predicted secondary and tertiary structure of ZmRab7, which was similar to the spatial structure of AtRab7. Furthermore, in the promoter region of ZmRab7, there were four GT1GMSCAM4 and two DRECRTCOREAT cis-acting elements to respond to salt stress. ZmRab7 was mainly expressed in the maize root and young embryo, and slightly repressed by high salt treatment. Additionally, heterologous expression of ZmRab7 in yeast remarkably reduced yeast cell tolerance to salt stress. Taken together, these results suggested that ZmRab7 might act as a negative regulator involved in the salt stress response.

Key words: Maize, Rab7, Small G protein, Salt stress

CLC Number:

Q78
S513.03

NIU Yanli, JIA Mingzhu, HAN Shuan, FU Jiamiao, LIU Lingyun. Cloning and Salt Stress Response Analysis of ZmRab7 Gene in Zea mays L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 1-6. doi: 10.7668/hbnxb.20190741.

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