RGA ANAlysis of the Wheat Leaf Rust Resistance Gene Lr35

doi:10.7668/hbnxb.2008.06.011

Abstract

Abstract: Based on the conserved domains (NBS,LRR etc) of the known resistance genes,primers were designed to amplify resistant homologous sequences in the near isogenic lines (NILs) of wheat leaf rust resistance using RGA method.A 747 bp specific fragment was found in TcLr35 by primer pair Pto-kin1IN/ XLRR-INV1.The specific fragment was isolated from the polyacrylamide gels,reamplified,cloned and sequenced. The result indicated that it has the high homology with the Triticum urartu clone BAC 210J 24, Triticum monococcum DV92 clone BAC 231A16 etc DNA fragmentas from wheat,which provide the shortcut for the cloning of wheat leaf rust resistance gene Lr35.

Key words: Wheat leaf rust disease, Resistance gene, Lr35, Reistance gene analogys

CLC Number:

S435.121.+3

GAO Qian, WANG Hai-yan, LIU Da-qun, LI Xing, YANG Wen-xiang. RGA ANAlysis of the Wheat Leaf Rust Resistance Gene Lr35[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 50-53. doi: 10.7668/hbnxb.2008.06.011.

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