Cloning and Sequence Analysis of a DNA Encoding Ethylene Receptor Etr2 from Chiyoda Strawberry

doi:10.7668/hbnxb.2008.06.010

Abstract

Abstract: In this study a ripening related ethylene receptor etr2 gene in strawberry fruits was cloned for the following-up research on gene funct ion and improving variet ies of strawberry through transgenic technology. A pair of primers were designed and synthesized based on the sequence reported of Chandler -Etr2. Using genome DNA extracted from Chinyoda strawberry mature fruits as template the specific PCR product of etr2 was obtained, then it was ligated to pGEM-T easy vector and sequenced. The sequencing data showed that the PCR product was 1 049 bp encoding 349 predicted amino acid residues. Comparison with the cDNA sequence from Chandler - etr2 indicated the homlogy was 99%, and amino acid identities were 98%.

Key words: Strawberry, Ethylene receptor, Gene clone, Sequence analysis

CLC Number:

S668.

SONG Chun-li, MA Jun-lian, TANG Xia, ZHANG Zi-de, LIU Yong-ju. Cloning and Sequence Analysis of a DNA Encoding Ethylene Receptor Etr2 from Chiyoda Strawberry[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 46-49. doi: 10.7668/hbnxb.2008.06.010.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2008.06.010

http://www.hbnxb.net/EN/Y2008/V23/I6/46

[1]	XIAO Shikui, LI Fang, ZHANG Wenting, LÜ Shufang, SHI Guoan, WU Jiang, FAN Bingyou. *Cloning and Prokaryotic Expression of ACS* Gene from Paeonia lactiflora Pall.** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 36-44.
[2]	CHENG Chunhong, YOU Jingfan, CAI Zhaoming, YE Chunhong, CHEN Li. *Cloning and Expression Analysis of BjuEAR1-1* in Tuber Mustard** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 37-43.
[3]	TANG Zhongli, LU Xiaonan, ZHAO Rui, LIU Qinghua, LI Meilan, LEI Fengjin, XU Xiaoyong. *Identification of Enzyme Genes Related to Carotenoid Synthesis and Cloning Analysis of PSY1* and LCYE2 in Yellow Peeled Zucchini** [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 60-67.
[4]	CHEN Longzheng, LIU Jing, LIU Zhiyang, XIA Pengfei, YUAN Xihan, NING Yu. Cloning and Expression Analysis of Powdery Mildew Responsive Gene McMLO1 in Momordica charantia [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 165-171.
[5]	LIAN Kaiqi, JI Shuang, ZHOU Lingling, SHI Zhiqi, WANG Fei, ZHANG Yuanchen. Cloning and Prokaryotic Expression of a clip-type Serine Protease Gene from Mythimna separate [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 195-201.
[6]	LI Qi, YANG Changheng, WANG Yong, LIN Yaqiu, XIANG Hua, ZHU Jiangjiang. Cloning and Analysis of Goat CIDEs and Identification of Predictive Interaction Proteins [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 211-221.
[7]	JIN Fengmei, XUE Jun, SUN Haibo, HAO Zhiyu. *Molecular Detection and Partial Genomic Sequence Analysis of Tomato mosaic virus* in Tianjin Based on Small RNA Sequencing** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(5): 176-183.
[8]	AERZUGULI Maimaitituerxun, ZHANG Zijun, ZHANG Weihong. *Complete Mitochondrial Genome Sequence of Oxyloma wujiaquensis* Li, Guo & Zhang, 2017(Gastropoda：Succineidae)** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 230-238.
[9]	HU Yueqing, WANG Ruozhong, HUANG Zhigang, XIAO Langtao. *Cloning and Prokaryotic Expression of Transcription Factor StWRKY57* Gene in Potato** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 10-17.
[10]	DONG Hui, YANG Li, LI Li, ZHANG Jianjun, FAN Jingfang, SONG Shijia, YANG Lei, LI Haishan. High Density Genetic Map of Cultivated Strawberry by SLAF-seq [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 52-57.
[11]	CAI Xiao, ZHEN Junbo, LIU Linlin, LIU Di, TANG Liyuan, ZHANG Sujun, LI Xinghe, WANG Haitao, LIU Cunjing, ZHANG Xiangyun, ZHANG Jianhong, CHI Jina. *Cloning and Expression Analysis of PP2C24* Gene from Gossypium arboreum L.** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 33-39.
[12]	SHEN Haoran, BAI Hui, REN Shilong, WANG Lu, WANG Yongfang, QUAN Jianzhang, DONG Zhiping, LI Zhiyong. *Detection and Variation Analysis of PWL Gene Family of Magnaporthe oryzae* in Foxtail Millet** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 178-183.
[13]	ZHANG Xi, QIAN Yuyuan, LIU Yi, WANG Guang, SONG Shijia, LI Xiaofei, MI Huanfang, CUI Shufang, LI Junlan. Cloning and Expression Analysis of a Cotton Pigment Gland Formation Related Gene GhNAC201 [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 29-35.
[14]	ZHEN Junbo, LIU Di, SONG Shijia, LIU Linlin, CAI Xiao, ZHANG Xi, LI Haishan, CHI Ji. Cloning and Expression Analysis of Two Late Embryogenesis Abundant(LEA) Proteins from Upland Cotton [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 33-38.
[15]	LIU Yufeng, ZHU Tianhui, LIU Yinggao, QIAO Tianmin, LI Shujiang, LONG Xumei, HAN Shan. *Cloning, Sequence Analysis and Prokaryotic Expression of CmWRKY* from Castanea mollissima BL** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(4): 37-45.

Please choose a citation manager

Content to export