Location and Resistance Analysis of Stripe Rust Resistance Gene YrCH7056 in Adult Plant Stage of Wheat

doi:10.7668/hbnxb.2018.01.004

Abstract

Abstract: Stripe rust resistance and genetic model from Thinopyrum ponticum(2n=10x=70) were studied to better utilize the resource and expand the selection range of resistance source for breeding in wheat. Recombinant inbred lines (RILs) were constructed by using CH7056, the introgression line of Th. ponticum, and wheat cultivars SY95-71. The F₇ and F₈ populations from RILs were identified for their adult plant resistance using mixed Pst types CYR32+CYR33+v26. The results showed that the proportion of resistant and susceptible individual plants in the genetic groups were both close to 1:1 in 2013 and 2014, suggesting that CH7056 carried one dominant stripe rust resistance gene,temporarily designated YrCH7056.Exogenous signals had not been detected using genomic in situ hybridization. YrCH7056 was located on wheat chromosome 1B by scaning DArT chip to the bulks of resistance and suspection of RILs.Then 129 pairs of the public SSR markers and 72 pairs of developed Thinopyrum ponticum -specific SSR markers on chromosome 1B were screened to construct the genetic map of YrCH7056. The flanking markers were 1BL-3848555 and barc240 with genetic distance of 1.1,2.5 cM,respectively.The stripe rust resistance gene differs from the resistance gene that had been mapped on chromosome 1BL by comparing the period of resistance,the source of gene,and the close linkage distance of the marker.It was deduced that YrCH7056 was a new gene of stripe rust resistance.In the meantime,the amplified bands of 1BL-3848555 among Thinopyrum ponticum, Thinopyrum ponticum -wheat octoploid Xiaoyan 7430 and CH7056 were the same,which showed that YrCH7056 might derive from Thinopyrum ponticum.

Key words: Wheat, Thinopyrum ponticum, Stripe rust, Adult plant resistance gene, Molecular mapping

CLC Number:

S435.1
Q78

ZHU Yan, QIAO Linyi, ZHANG Xiaojun, LI Xin, YANG Zujun, GUO Huijuan, WANG Changbiao, CHANG Zhijian. Location and Resistance Analysis of Stripe Rust Resistance Gene YrCH7056 in Adult Plant Stage of Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(1): 20-24. doi: 10.7668/hbnxb.2018.01.004.

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