Development of Molecular Markers Linked to a Novel QTL for TuMV-C4 Resistance in Brassica rapa L.ssp. pekinensis

doi:10.7668/hbnxb.2014.06.001

Abstract

Abstract: Our research constructed a molecular genetic map with a line 91-112 (highly resistant to TuMV-C4),a highly susceptible line T12-19 and the DH population derived from microspore culture of F₁ (91-112×T12-19).A total of 45 markers were mapped to the A09 chromosome,and a new QTL-BrTuA09 controlling TuMV-C4 resistance was identified.On this basis,27 pairs of primers were designed by screening the InDel loci on A09 chromosome of Chinese cabbage based on genome re-sequencing.The results showed that distinct PCR amplification products were obtained from the InDel markers.Among these markers,11 InDel were polymorphic on the tested parent materials,and mapped to the A09 chromosome.By linkage analysis,these markers were tightly linked to the QTL-BrtuA09.The studies showed that these markers can be applied in marker-assisted selection in Chinese cabbage hybrid breeding programs and improve the resistance to TuMV-C4.

Key words: Chinese cabbage, QTL, TuMV, Molecular markers

CLC Number:

Q78
S634.3

TIAN Xi-hui, YU Shuan-cang, SU Tong-bing, ZHANG Feng-lan, YU Yang-jun, ZHANG De-shuang, ZHAO Xiu-yun, WANG Wei-hong. Development of Molecular Markers Linked to a Novel QTL for TuMV-C4 Resistance in Brassica rapa L.ssp. pekinensis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(6): 1-5. doi: 10.7668/hbnxb.2014.06.001.

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References

[1] 李树德.中国主要蔬菜抗病育种进展[M].北京:科学出版社,1995:26-29.
[2] 冯兰香,徐玲,刘佳,等.北京地区大白菜芜菁花叶病毒株系的鉴定[J].中国蔬菜,1988(4):23-25.
[3] 谭其猛.蔬菜育种[M].北京:农业出版社,1980.
[4] 钮心恪.大白菜抗霜霉病、病毒病原始材料的筛选及抗性遗传的研究[J].中国蔬菜,1984(4):28-32.
[5] Leung H I,Williams P H.Cytoplasmic male sterile Brassica campestris breeding lines with resistance to clubroot,turnip mosaic,and downy mildew[J].Hort Science,1983,18(5):774-775.
[6] Yoon J Y,Green S K,Opena R T.Inheritance of resistance to turnip mosaic virus in Chinese cabbage[J].Euphytica,1993,69(1/2):103-108.
[7] 鹿英杰,康永春,李光池,等.大白菜对芜菁花叶病毒抗性遗传规律的研究[J].黑龙江农业科学,1988(6):27-31.
[8] 潘春清.大白菜 AFLP 遗传图谱构建及抗 TuMV-C3 的 QTL 分析[D].哈尔滨:东北农业大学,2007.
[9] 李巧云,张晓亮,张志刚,等.大白菜分子遗传图谱构建研究进展[J].生物技术通报,2012(6):18-24.
[10] Rusholme R L.The genetic control of resistance to turnip mosaic virus (TuMV) in Brassica[D].Norwich:University of East Anglia (PhD Thesis,),2000.
[11] Rusholme R L,Higgins E E,Walsh J A,et al.Genetic control of broad-spectrum resistance to turnip mosaic virus in Brassica rapa (Chinese cabbage)[J].Journal of General Virology,2007,88(11):3177-3186.
[12] Fujiwara A,Inukai T,Kim B M,et al.Combinations of a host resistance gene and the CI gene of turnip mosaic virus differentially regulate symptom expression in Brassica rapa cultivars[J].Archives of Virology,2011,156(9):1575-1581.
[13] Jin M A,Lee S S,Ke L,et al.Identification and mapping of a novel dominant resistance gene,TuRB07 to turnip mosaic virus in Brassica rapa [J].Theoretical and Applied Genetics,2014,127(2):509-519.
[14] 屈淑平,张彤,张俊华,等.大白菜抗TuMV-C3的QTL分析[J].东北农业大学学报,2009,40(11):33-37.
[15] 韩和平,孙日飞,张淑江,等.大白菜中与芜菁花叶病毒(TuMV)感病基因连锁的AFLP标记[J].中国农业科学,2004,37(4):539-544.
[16] 张俊华,屈淑平,崔崇士.大白菜抗芜菁花叶病毒的QTL分析[J].植物病理学报,2008,38(2):178-184.
[17] 张晓伟,原玉香,王晓武,等.大白菜DH群体TuMV抗性的QTL定位与分析[J].园艺学报,2009,36(5):731-736.
[18] Zhang F L,Wang M,Liu X C,et al.Quantitative trait loci analysis for resistance against Turnip mosaic virus based on a doubled-haploid population in Chinese cabbage[J].Plant Breeding,2008,127(1):82-86.
[19] Ooijen J W,Voorrips R E.JoinMap:version 3.0 software for the calculation of genetic linkage maps[M].Wageningen:Wageningen University and Research Center,2001.
[20] Yu S C,Zhang F L,Zhao X,et al.An improved Brassica rapa genetic linkage map and locus-specific variations in a doubled haploid population[J].Plant Molecular Biology Reporter,2013,31(3):558-568.
[21] Kosambi D D.The estimation of map distances from recombination values[J].Annals of Eugenics,1943,12(1):172-175.
[22] Qian W,Zhang S J,Zhang S F,et al.Mapping and candidate-gene screening of the novel Turnip mosaic virus resistance gene retr02 in Chinese cabbage (Brassica rapa L.)[J].Theoretical and Applied Genetics,2013,126(1):179-188.

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