Genetic Analysis and Gene Fine Mapping of a Midrib-deficient Mutant dl-6 in Rice

doi:10.7668/hbnxb.2014.06.002

Abstract

Abstract: To clone the DL-6 locus,positive and negative cross combinations were developed between dl-6 and 9311,and genetic analysis found that drooping leaf was controlled by one recessive nuclear gene DL-6. DL-6 was primarily mapped on the short arm of chromosome 3 with SSR markers.With newly developed markers DL-6 was finally fine mapped in 85 kb region between markers I3-5 and I3-8.Open reading frame (ORF) analysis revealed that ORF9 might be a drooping leaf related gene.ORF9 both in mutant dl-6 and in wild type were sequenced and 1 bp mutation was found in first exon in dl-6 when blasted with Nipponbare.Cysteine mutated into arginine in dl-6.Meanwhile,8 bp deletion was also found at 3' end of YABBY.These two mutations which one is the functional mutation of DL-6 is still uncertain and need deeply research.

Key words: Rice, Midrib-deficient, Gene mapping, dl-6

CLC Number:

Q78
S511.03

WANG Jun, WANG Jing, YANG Jie, ZHAO Xiang-qiang, ZHU Jin-yan, FAN Fang-jun, LI Wen-qi, WANG Fang-quan, ZHONG Wei-gong. Genetic Analysis and Gene Fine Mapping of a Midrib-deficient Mutant dl-6 in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(6): 6-10. doi: 10.7668/hbnxb.2014.06.002.

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