Improving Blast Resistance of Rice Restorer 389 and Its Hybrid Through Molecular Marker-assisted Selection

doi:10.7668/hbnxb.2018.02.016

Abstract

Abstract: To directionally improve blast resistance of the indica rice restorer R389 and its hybrid,a functional DNA marker Ins1-1 was developed according to the broad-spectrum and durable blast resistance gene Pi9, and molecular marker-assisted selection(MAS) consecutive backcross breeding were implemented. Resistance responses and resistance spectra to twenty tested M.oryzae isolates were quite different between the Pi9 gene donor 75-1-127 conferring resistance to eighteen isolates and the receptor R389 which showed resistance to only eleven isolates,and the resistance frequencies were 90.0% and 55.0%,respectively in the greenhouse inoculation assay. Accordingly,75-1-127 showed high-level blast resistance during seedling stage and panicle developing stage but R389 was highly susceptible in field nursery. Ins1-1 was a PCR-based dominant DNA marker,showed obvious and stable polymorphism between two parents,by amplifying a 513 bp band from the genome of donor 75-1-127,the pure line of R389-Pi9 and its hybrid,but no product from that of the receptor R389 and its hybrid. Selection efficiency by Ins1-1 genotype for blast resistance phenotype reached 100%. An improved restorer line R389-Pi9 was bred from the twenty-six BC₆F₃ family lines,which showed similar field characteristics but much better blast resistance comparing to R389,and its two-line hybrid Liangyou 389-Pi9(P88s/R389-Pi9) was created which showed high level seedling and panicle blast resistance in field nursery. Field cultivation assay showed that the improved pure line R389-Pi9 and its hybrid Liangyou 389-Pi9 showed no significant difference to corresponding contrasts in main agronomic traits such as growth period,plant height,seed setting rate and 1000-grain weight,implying similar genetic background and improved blast resistance were achieved.

Key words: Rice, Blast resistance improvement, The Pi9 gene, Molecular marker-assisted selection

CLC Number:

LI Yongcong, HUANG Jun, LIAO Hua, CHEN Hailong, YANG Fengyu, LAI Yifan, ZHANG Xuhui, LIU Jinling, WANG Guoliang, LIU Xionglun. Improving Blast Resistance of Rice Restorer 389 and Its Hybrid Through Molecular Marker-assisted Selection[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(2): 112-118. doi: 10.7668/hbnxb.2018.02.016.

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