Effect of Application of Bioorganic Fertilizer on Tomatoes Fruit Quality and Soil Biological Characteristics

doi:10.7668/hbnxb.20191308

Abstract

Abstract: To ascertain the effect of applying bio-organic fertilizer for 4 consecutive years on tomato fruit and soil biological characteristics, physiological and biochemical methods and high-throughput sequencing technology were combined to analyze its regulation effect on tomato fruit quality, soil nutrient content and microbial community structure.The results showed that,with chemical fertilizer replaced by bioorganic fertilizer, the sugar acid ratio, content of Vc and lycopene of tomatoes were significantly increased by 11.1%, 7.2% and 12.4% respectively. Soil nutrient measurement exhibited that, the high-efficiency nitrogen-fixing bacteria contained in bioorganic fertilizer could meet the nitrogen demand of tomato growth, and soil available phosphorus, available potassium and organic matter content were significantly increased by 147.0%, 38.8% and 35.6% respectively. High-throughput sequencing showed that, application of bioorganic fertilizer for four consecutive years could improve the relative abundance of soil Streptomyces, Ureibacillus and Bacillus, among which the activation effect on B. asahii was most significant. In addition,Coverage and Simpson index of tomato-planted soil microbial treated by bioorganic fertilizer for four consecutive years were both higher than those treated by common chemical fertilizer, which indicated that the bioorganic fertilizer containing high-efficiency nitrogen-fixing bacteria could improve soil microbe coverage and dominance to some extent. Obviously, continuous application of bioorganic fertilizer is helpful to improve tomatoes fruit quality, soil fertility and soil micro-ecological environment.

Key words: Tomatoes quality, Bioorganic fertilizer, Soil nutrient, Microbe diversity, Soil improvement

CLC Number:

S141

JIANG Lili, WANG Kaiyun, WU Yuguo, WANG Kaiyuan, WANG Hongyan. Effect of Application of Bioorganic Fertilizer on Tomatoes Fruit Quality and Soil Biological Characteristics[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 141-147. doi: 10.7668/hbnxb.20191308.

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References

[1] 许景钢, 孙涛, 李嵩. 我国微生物肥料的研发及其在农业生产中的应用[J]. 作物杂志, 2016(1):1-6.doi:10.16035/j.issn.1001-7283.2016.01.001.
Xu J G, Sun T, Li S. Application of microbial fertilizers in agricultural production of China[J]. Crops, 2016(1):1-6.
[2] 曹丹, 宗良纲, 肖峻,张倩, 赵妍. 生物肥对有机黄瓜生长及土壤生物学特性的影响[J]. 应用生态学报, 2010, 21(10):2587-2592.doi:10.13287/j.1001-9332.2010.0378.
Cao D, Zong L G, Xiao J, Zhang Q, Zhao Y. Effect of bio-fertilizer on organically cultured cucumber growth and soil biological characteristics[J]. Chinese Journal of Applied Ecology, 2010, 21(10):2587-2592.
[3] 姜莉莉, 王开运, 武玉国, 王开元, 王洪祥. 生物有机肥在玫瑰香葡萄上的应用效果[J]. 落叶果树, 2019, 51(1):18-21.doi:10.13855/j.cnki.lygs.2019.01.005.
Jiang L L, Wang K Y, Wu Y G, Wang K Y, Wang H X. Application efficacy of bioorganic fertilizer on muscat grape[J]. Deciduous Fruits, 2019, 51(1):18-21.
[4] 李红峥, 曹红霞, 郭莉杰, 吴宣毅. 沟灌方式和灌水量对温室番茄综合品质与产量的影响[J]. 中国农业科学, 2016, 49(21):4179-4191.doi:10.3864/j.issn.0578-1752.2016.21.012.
Li H Z, Cao H X, Guo L J, Wu X Y. Effect of furrow irrigation pattern and irrigation amount on comprehensive quality and yield of greenhouse tomato[J]. Scientia Agricultura Sinica, 2016, 49(21):4179-4191.
[5] 马宏飞, 卢生有, 韩秋菊, 李薇. 紫外分光光度法测定五种果蔬中维生素C的含量[J]. 化学与生物工程, 2012, 29(8):92-94.doi:10.3969/j.issn.1672-5425.2012.08.026.
Ma H F, Lu S Y, Han Q J, Li W. Determination of vitamin C content in five kinds of fruits and vegetables by UV spectrophotometry[J]. Chemistry & Bioengineering, 2012, 29(8):92-94.
[6] 豆峻岭, 刘文革, 赵胜杰, 路绪强, 何楠, 朱红菊, 高磊. 三倍体无籽西瓜果实发育期番茄红素合成代谢酶基因的表达[J]. 果树学报, 2014, 31(4):589-595.doi:10.13925/j.cnki.gsxb.20140056.
Dou J L, Liu W G, Zhao S J, Lu X Q, He N, Zhu H J, Gao L. Expression of lycopene synthesis and metabolism genes during maturation of triploid seedless watermelon[J]. Journal of Fruit Science, 2014, 31(4):589-595.
[7] 中国科学院南京土壤研究所. 土壤理化分析[M]. 上海:上海科学技术出版社, 1978:62-142.
Nanjing Institute of Soil Science.Soil chemical and physicalanalysis[M].Shanghai:Shanghai Science and Technology Press, 1978:62-142.
[8] Sułowicz S, Piotrowska-Seget Z. Response of microbial communities from an apple orchard and grassland soils to the first-time application of the fungicide tetraconazole[J]. Ecotoxicology and Environmental Safety, 2016, 124:193-201.doi:10.1016/j.ecoenv.2015.10.025.
[9] 许云翔, 何莉莉, 刘玉学, 吕豪豪, 汪玉瑛, 陈金媛, 杨生茂. 施用生物炭6年后对稻田土壤酶活性及肥力的影响[J]. 应用生态学报, 2019, 30(4):1110-1118.doi:10.13287/j.1001-9332.201904.002.
Xu Y X, He L L, Liu Y X, Lü H H, Wang Y Y, Chen J Y, Yang S M. Effects of biochar addition on enzyme activity and fertility in paddy soil after six years[J]. Chinese Journal of Applied Ecology, 2019, 30(4):1110-1118.
[10] 朱丹,韦泽秀,刘晓燕,周志峰,代先祝,王晓锋,夏志强,吴先勤,蔚建军,付莉,张磊. 菌肥对青稞根际土壤理化性质以及微生物群落的影响[J]. 土壤学报, 2014, 51(3):627-637.doi:10.11766/trxb201311030512.
Zhu D, Wei Z X,Liu X Y,Zhou Z F, Dai X Z, Wang X F, Xia Z Q, Wu X Q, Yu J J, Fu L, Zhang L. Effects of bacterial manure on soil physicochemical properties and microbial community diversity in rhizosphere of highland barly[J]. Acta Pedologica Sinica, 2014, 51(3):627-637.
[11] 库永丽, 徐国益, 赵骅, 董天旺, 曹翠玲. 微生物肥料对猕猴桃高龄果园土壤改良和果实品质的影响[J]. 应用生态学报, 2018, 29(8):2532-2540.doi:10.13287/j.1001-9332.201808.025.
Ku Y L, Xu G Y, Zhao H, Dong T W, Cao C L. Effects of microbial fertilizer on soil improvement and fruit quality of kiwifruit in old orchard[J]. Chinese Journal of Applied Ecology, 2018, 29(8):2532-2540.
[12] 陈龙, 孙广正, 姚拓, 柴强, 康文娟, 蒋永梅. 干旱区微生物肥料替代部分化肥对玉米生长及土壤微生物的影响[J]. 干旱区资源与环境, 2016, 30(7):108-113.doi:10.13448/j.cnki.jalre.2016.224.
Chen L, Sun G Z, Yao T, Chai Q, Kang W J, Jiang Y M. Effect of chemical fertilizer partly replaced by microbial fertilizer on maize growth and soil microorganism in arid area[J]. Journal of Arid Land Resources and Environment, 2016, 30(7):108-113.
[13] 王文庆, 史清亮, 白建军, 刘平, 张玉花. 微生物肥料对山药土壤生态特征及病情指数的影响[J]. 山西农业科学, 2010, 38(12):37-39, 56.doi:10.3969/j.issn.1002-2481.2010.12.12.
Wang W Q, Shi Q L, Bai J J, Liu P, Zhang Y H. Effects of microbial fertilizers on the ecological characteristics of soil in the Chinese yam field and disease index[J]. Journal of Shanxi Agricultural Sciences, 2010, 38(12):37-39, 56.
[14] Li R, Tao R, Ling N, Chu G X. Chemical, organic and bio-fertilizer management practices effect on soil physicochemical property and antagonistic bacteria abundance of a cotton field:implications for soil biological quality[J]. Soil and Tillage Research,2017, 167:30-38.doi:10.1016/j.still.2016.11.001.
[15] 姜莉莉, 武玉国, 王开运. 丰田宝微生物菌肥在温室番茄上的应用效果[J]. 农业科技通讯, 2015, 12:171-172, 284.doi:10.3969/j.issn.1000-6400.2015.12.062.
Jiang L L, Wu Y G, Wang K Y. Application effect of Fengtianbao microbial fertilizer on greenhouse tomatoes[J]. Bulletin of Agricultural Science and Technology, 2015, 12:171-172, 284.
[16] 燕红, 于彩莲, 包鑫, 乔晓飞. 高效解磷兼解钾活性菌株分离筛选的初步研究[J]. 扬州大学学报(农业与生命科学版), 2016, 37(1):81-85, 90.doi:10.16872/j.cnki.1671-4652.2016.01.016.
Yan H, Yu C L, Bao X, Qiao X F. Preliminary study on isolation and screening of highly-effective strain in decomposing phosphate and potassium[J]. Journal of Yangzhou University (Agricultural and Life Science Edition), 2016, 37(1):81-85, 90.
[17] 李伟成, 盛海燕, 陈伟杰, 刘姚姚, 张瑞, 温星. 毛竹入侵马尾松林的土壤菌群多样性变化[J]. 应用生态学报, 2018, 29(12):3969-3976.doi:10.13287/j.1001-9332.201812.002.
Li W C, Sheng H Y, Chen W J, Liu Y Y, Zhang R, Wen X. Variation of soil bacterial diversity after the invasion of Phyllostachys edulis in Pinus massoniana forest[J]. Chinese Journal of Applied Ecology, 2018, 29(12):3969-3976.
[18] 任天宝, 杨艳东, 高卫锴, 李宙文, 阎海涛, 王省伟, 刘国顺. 基于高通量测序的生物炭施用量对植烟土壤细菌群落的影响[J]. 河南农业科学, 2018, 47(12):64-69.doi:10.15933/j.cnki.1004-3268.2018.12.010.
Ren T B, Yang Y D, Gao W K, Li Z W, Yan H T, Wang S W, Liu G S. Effects of application amount of biochar on soil bacterial community in tobacco fields based on high-throughput sequencing[J]. Journal of Henan Agricultural Sciences, 2018, 47(12):64-69.
[19] 艾超, 孙静文, 王秀斌, 梁国庆, 何萍, 周卫. 植物根际沉积与土壤微生物关系研究进展[J]. 植物营养与肥料学报, 2015, 21(5):1343-1351.doi:10.11674/zwyf.2015.0530.
Ai C, Sun J W, Wang X B, Liang G Q, He P, Zhou W. Advances in the study of the relationship between plant rhizodeposition and soil microorganism[J]. Journal of Plant Nutrition and Fertilizers, 2015, 21(5):1343-1351.
[20] 赵卫松, 郭庆港, 李社增, 王培培, 鹿秀云, 苏振贺, 张晓云, 马平. 花铃期棉花黄萎病抗病与感病品种对土壤细菌群落结构的影响[J]. 中国农业科学, 2020, 53(5):942-954.doi:10.3864/j.issn.0578-1752.2020.05.007.
Zhao W S, Guo Q G, Li S Z, Wang P P, Lu X Y, Su Z H, Zhang X Y, Ma P. Effect of wilt-resistant and wilt-susceptible cotton on soil bacterial community structure at flowering and boll stage[J]. Scientia Agricultura Sinica, 2020, 53(5):942-954.
[21] 黄金枝, 胡桂萍, 俞燕芳, 杜贤明, 石旭平, 王军文. 微生物在农业废弃物堆肥应用中的研究进展[J]. 广东农业科学, 2019, 46(1):64-70.doi:10.16768/j.issn.1004-874X.2019.01.010.
Huang J Z, Hu G P, Yu Y F, Du X M, Shi X P, Wang J W. Research progress of microbe in agricultural waste composting[J]. Guangdong Agricultural Sciences, 2019, 46(1):64-70.
[22] 刘岳飞, 吴人敏, 张传进, 姚华开, 杨尚东. 基于传统和高通量测序技术研究地膜对辣椒土壤特性及细菌多样性的影响[J]. 土壤学报, 2019, 56(4):986-993.doi:10.11766/trxb201810100018.
Liu Y F, Wu R M, Zhang C J, Yao H K, Yang S D. Effect of different mulching treatments on soil properties and bacterial diversity in pepper fields[J]. Acta Pedologica Sinica, 2019, 56(4):2019, 56(4):986-993.
[23] 段倩倩, 杨晓红, 黄先智. 石漠化地区生态桑林根际土壤微生物资源多样性研究[J]. 西南大学学报(自然科学版), 2017, 39(7):25-29.doi:10.13718/j.cnki.xdzk.2017.07.004.
Duan Q Q, Yang X H, Huang X Z. The diversity of microbial resources in ecological mulberry rhizosphere soil in a rocky desertification area[J]. Journal of Southwest University (National Science Edition), 2017, 39(7):25-29.
[24] Wang M P, Chen L, Li Y T, Chen L, Liu Z Y, Wang X J, Yan P S, Qin S. Responses of soil microbial communities to a short-term application of seaweed fertilizer revealed by deep amplicon sequencing[J]. Applied Soil Ecology, 2018, 125:288-296.doi:10.1016/j.apsoil.2018.02.013.
[25] Feng Y Z, Chen R R, Hu J L, Zhao F, Wang J H, Chu H Y, Zhang J B, Dolfing J, Lin X G. Bacillus asahii comes to the fore in organic manure fertilized alkaline soils[J]. Soil Biology and Biochemistry, 2015, 81:186-194.doi:10.1016/j.soilbio.2014.11.021.
[26] 郭丽丽, 尹伟伦, 郭大龙, 侯小改. 油用凤丹牡丹不同种植时间根际细菌群落多样性变化[J]. 林业科学, 2017, 53(11):131-141.doi:10.11707/j.1001-7488.20171115.
Guo L L, Yin W L, Guo D L, Hou X G. Variations of bacterial biodiversity in rhizosphere soils of oil tree peony cropping continuously for different years[J]. Scientia Silvae Sinicae, 2017, 53(11):131-141.
[27] 牛世全, 龙洋, 李海云, 达文燕, 胡山, 李渭娟, 朱学泰, 孔维宝.应用IlluminaMiSeq高通量测序技术分析河西走廊地区盐碱土壤微生物多样性[J]. 微生物学通报, 2017, 44(9):2067-2078.doi:10.13344/j.microbiol.china.160824.
Niu S Q, Long Y, Li H Y, Da W Y, Hu S, Li W J, Zhu X T, Kong W B. Microbial diversity in saline alkali soil from Hexi Corridor analyzed by Illumina Miseq high-throughput sequencing system[J]. Micobiology, 2017, 44(9):2067-2078.
[28] 陈孟立, 曾全超, 黄懿梅, 倪银霞. 黄土丘陵区退耕还林还草对土壤细菌群落结构的影响[J]. 环境科学, 2018, 39(4):1824-1832.doi:10.13227/j.hjkx.201708090.
Chen M L, Zeng Q C, Huang Y M, Ni Y X. Effects of the farmland-to-forest/grassland conversion program on the soil bacterial community in the loess hilly region[J]. Environmental Science, 2018, 39(4):1824-1832.
[29] 叶雯, 李永春, 喻卫武, 叶晓明, 钱宇汀, 戴文圣. 不同种植年限香榧根际土壤微生物多样性[J]. 应用生态学报, 2018, 29(11):3783-3792.doi:10.13287/j.1001-9332.201811.034.
Ye W, Li Y C, Yu W W, Ye X M, Qian Y T, Dai W S. Microbial biodiversity in rhizospheric soil of Torreya grandis ‘Merrillii’ relative to cultivation history[J]. Chinese Journal of Applied Ecology, 2018, 29(11):3783-3792.

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