Molecular Improvement Based on the Strategy of ‘Multiple Genes Pyramiding-early-generation Hybridized Combination’ in Rice

doi:10.7668/hbnxb.2017.03.012

Abstract

Abstract: Low selection-efficient and long cycle are the main bottle necks of conventional breeding technique.In order to make improvement of the efficiency of variety breeding and hybridized-combination,we innovated the molecular breeding strategy in rice.Then we carried out molecular improvement of restorer line and evaluation of new hybrid combination by multiple genes pyramiding and early-generation hybridized combination.The donor parent H318 was hybridized with restorer parent Huazhan,and we constructed the multiple genes pyramiding technology by selection and design molecular markers,and performed pyramiding-improvement application with 6 functional genes for grain quality,fragrance,and disease resistance,including Wx^b,fgr,Xa23,Pi2,Pi46,and Pita.As the result,we achieved a series of 14 stable rice lines with genetic background of restorer line Huazhan,homozygous target-genes,and high grain-quality,double-resistant,and fragrance.In accordance with stable-inheritance properties,ideas of early-generation hybridized combination was raised.Test cross of improved lines with CMS lines in production practice of NingA and C815S were carried out,and performance of new hybrid combination was evaluated,therefore,potential superior hybrid rice was screened and achieved.In this study,breeding programs adopted the strategy of multiple genes pyramiding-early-generation hybridized combination,this method could realize the quick pyramiding of multiple favorable genes,targeted improvement of key traits of grain quality and disease-resistant,promotion of high-efficiency breeding of hybrid rice.

Key words: Rice, Molecular improvement, Genes pyramiding, Hybridized combination, Restorer line

CLC Number:

CHEN Likai, GUO Tao, LIU Yongzhu, WANG Hui, CHEN Zhiqiang. Molecular Improvement Based on the Strategy of ‘Multiple Genes Pyramiding-early-generation Hybridized Combination’ in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(3): 77-84. doi: 10.7668/hbnxb.2017.03.012.

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