Abstract: Normally, a single 10-mer oligonucleotide primer is used in a RAPD amplification. However, if pairs of two primers are used, theoretically more fragments should be amplified. In this experiment, we used 140 decamer primers to screen molecular markers specific for individual radish chromosomes in Raphanobrassica, both individually and in pairs (here named dpRAPD) with three fluorescence-labelled primers: OPD11FAM, OPG19Tet and OPH15Hex. On the average, each single primer generated 1.3 chromosome-specific markers in RAPD, while each pairwise combination of primers generated 1.7 markers in dpRAPD. The percentage of excellent and good bands in dpRAPD (78% ) was higher than for RAPD (75%). It was found that the majority of dpRAPD bands are different from original RAPD bands. Fragments with length under 100 bp which are difficult to be identified by polyacrylamide or agarose gel electrophoresis are detectable using Genetic Analyzer. It was shown that dpRAPD is a useful complement to classical RAPD analysis.

Key words: RAPD, dp RAPD, Polyacrylamide gel electrophoresis, Silver staining

CLC Number: 

• S565.4