Genetic PolymoRphism of 20 Sweet SoRghum CultivaRs as Revealed by RAPD and SSR MaRkeRs

doi:10.3969/j.issn.1000-7091.2013.01.003

Abstract

Abstract: This study analyzed the genetic polymoRphism and Relationship of 20 sweet soRghum cultivaRs by RAPD and SSR moleculaR maRkeRs． The Results showed that 31 polymoRphism pRimeRs weRe obtained fRom the scReening using RAPD maRkeR analysis． A total of 202 bands weRe obtained; 167 of them showed polymoRphisms， with a polymoRphism Rate of 82． 67%; the genetic similaRity( GS) coefficients among the studied cultivaRs Ranged fRom 0． 613 to 0． 870; the aveRage genetic similaRity coefficient was 0． 744． A total of 297 bands weRe amplified fRom these 91 paiRs of SSR pRimeRs，and 267 bands showed polymoRphisms，with a polymoRphism Rate of 89． 90%，and genetic similaRity coefficients Ranging fRom 0． 555 to 0． 837 among the 20 sweet soRghum vaRieties weRe obtained，and the aveRage value was 0． 695． This Result displayed a similaR tRend with Results fRom the 2 individual data analyses discussed above foR RAPD and SSR moleculaR maRkeRs，with some diffeRences． The Results based on combined data could moRe compRehensively and objectively Reflect the fundamental genetic diffeRences among these vaRieties．

Key words: Sweet soRghum, ClusteR analysis, SSR, RAPD, PolymoRphism

CLC Number:

S514．03

XU Ying, ZHAN Qiu-wen, DONG Shu-jun, LI Jie-qin, LIN Ping. Genetic PolymoRphism of 20 Sweet SoRghum CultivaRs as Revealed by RAPD and SSR MaRkeRs[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(1): 12-18. doi: 10.3969/j.issn.1000-7091.2013.01.003.

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References

[1] 卢庆善．甜高粱[M]．北京: 中国农业科学技术出版社， 2008: 1 - 25．

[2] 李南珠，李玥莹． RAPD 分子标记技术在甜高粱基因组研究中的应用[J]．安徽农业科学， 2009， 37( 15) : 6881 - 6883， 6886．

[3] 李莹，刘莹，陶思源． RAPD 技术在甜高粱品种基因组分析上的应用[J]．沈阳师范大学学报，2007，25 ( 4) : 499 - 502．

[4] 谭雪，郭爽，郑少薇，等．菜薹种质资源亲缘关系的 RAPD 分析[J]．华北农学报， 2011， 26( 增刊) : 18 - 22．

[5] 蒋建平，杨懋勋，黄永芳．广东省野百合天然居群的随机扩增多态性DNA( RAPD) 分析[J]．华北农学报， 2011， 26( 4) : 130 - 134．

[6] 崔莹莹，郭安平，王晓玲，等． RAPD 和SSR 标记检测海南普通野生稻籼粳分化的比较研究[J]．热带作物学报， 2009， 30( 6) : 773 - 780．

[7] 邱红波，彭忠华，张文龙．利用RAPD 和SSR 分析36 个贵州主要玉米种质的比较研究[J]．种子，2009，28 ( 12) : 39 - 43．

[8] 詹秋文，李杰勤，汪保华，等． 42 份高粱与苏丹草及其2 个杂交种DNA 指纹图谱的构建[J]．草业学报， 2008， 17( 6) : 85 - 92．

[9] 骈跃斌，许晶，武岩军，等． SSR 分子标记技术在玉米杂种优势群划分中的应用[J]．山西农业科学，2012， 40( 5) : 439 - 441， 444．

[10] 柴美清，原佳敏． SSR 分子标记在玉米种质研究中的应用[J]．山西农业科学， 2011， 39( 9) : 1017 - 1019．

[11] 乔军，王利英，石瑶．利用SSR 进行品种纯度鉴定的研究进展[J]．天津农业科学， 2011， 17( 4) : 45 - 51．

[12] 张庶，周新成，李利斌，等．利用EST-SSR 标记鉴定大白菜杂交种纯度的研究[J]．天津农业科学， 2010， 16( 6) : 1 - 4．

[13] Yang W P，de OliveiRa A C，Godwin I． CompaRison of DNA maRkeR technologies in chaRacteRizing plant genome diveRsity: vaRiability in Chinese soRghums [J]． CRop Sci， 1996， 36: 1669 - 1676．

[14] Zhan Q W，Zhang T Z，Wang B H， et al． DiveRsity CompaRison and Phylogenetic Relationships of S． bicoloR and S． sudanense as Revealed by SSR MaRkeRs[J]． Plant Science， 2008， 174( 1) : 9 - 16．

[15] Rohlf F J． NTSYS-pc，NumeRical Taxonomy and MultivaRiate Analysis System，VeRsion 2． 10e[CP]． Applied Biostatistics Inc，New YoRk， 2000．

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