cDNA-RAPD Analysis on Genes during Flower Differentiation and Development of the Summer Buds of Grapevine

doi:10.7668/hbnxb.2011.02.010

Abstract

Abstract: In order to initiate some investigation of the molecular mechanism of flowering in grapevine,differential expression profile of cDNA between the summer buds on the shoots after cut back and those of CK was analyzed by cDNA-RAPD.Ten pairs of primer were screened used for the cDNA-RAPD amplification.Totally,153(81 differentially expressed fragments)fragments were detected from the grapevine genomic DNA-and cDNA-RAPD fingerprints that could be classed into 3 types.Gene expression of the treatments had some change,and the majority of differentially expressed genes showed increased expression.Fragments with obvious difference were selected to re-amplific ation and purification,sequenced,and 14 sequences were obtained.Most of them could be blasted at various levels wjth the sequence deposited in GenBank database.It showed that the cDNA-RAPD technology can be an preferential method for study on genes' differential expression.

Key words: Grapevine, Summer buds, Differential expression, cDNA-RAPD

CLC Number:

GUO Lei, WANG Chen, CAO Xue, YANG Guang, MU Qian, FANG Jing-gui. cDNA-RAPD Analysis on Genes during Flower Differentiation and Development of the Summer Buds of Grapevine[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 43-48. doi: 10.7668/hbnxb.2011.02.010.

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