Cloning and Expression Analysis of Differential Genes Between C-Type Cytoplasmic Sterile Line and Its Maintainer Line in Maize

doi:10.7668/hbnxb.2014.03.003

Abstract

Abstract: orf118-b and cox2^* were cloned from sterile line C48-2 and it maintainer line N48-2 in maize by PCR and RT-PCR.orf118-b is a specific chimeric open reading frame in mitochondria genome of CMS-C line. cox2^* was special transcript of cox2 only in N48-2, based on the maize genomic database, 5'UTR region of the transcript was extended comparison to the existed annotation.Expression of cdpk between C-Type cytoplasmic male sterility line and maintainer line at the pollen mother cells(PMCs) stage, the tetrad stage, the uninucleate stage and the binuclear stage were determined using Real-Time PCR.The results showed that cdpk was up-regulated in C48-2 at mother cell stage, tetrad stage, the binuclear stage, but was down-regulated in C48-2 at binuclear uninucleate stage.This results provide foundation for further explaining molecular mechanism of CMS-C in maize.

Key words: Maize, CMS-C, Chimeric ORF, Differential expression

CLC Number:

S513.03

WANG Ji-yue, YI Hong-yang, WANG Sheng-qing, CAO Mo-ju. Cloning and Expression Analysis of Differential Genes Between C-Type Cytoplasmic Sterile Line and Its Maintainer Line in Maize[J]. journal1, 2014, 29(3): 11-15. doi: 10.7668/hbnxb.2014.03.003.

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