Mapping QTL and Analysis on Glucosinolates Total Content in Cabbage

doi:10.7668/hbnxb.2014.03.002

Abstract

Abstract: Glucosinolates, anticancer active substances, are widely present in Brassica crops.It will be of important significance to carry out the breeding of high content of Glucosinolates new varieties of cabbage.This study analyzed total glucosinolate content of F₂ mapping population parents and that of 83 individual plants by HPLC methods.Based on the cabbage high-density genetic linkage map which had been constructed previously, QTL mapping and analysis related to Glucosinolates total content in the individuals of F₂ were performed using MapQTL 4.0.Three QTLs(qGS-7-1, qGS-8-1, qGS-8-2) located on LG7 and LG8 controlling the traits of Glucosinolates total content in cabbage were detected.The contribution rate were 13.6%, 7.5% and 5.6%, respectively.Meanwhile, two molecular markers(me27-em25-2, E38M55-7) coseparating with the QTLs could be useful for marker assisted selection of cabbage breeding rich in Glucosinolates.

Key words: Brassica oleracea var. capitata, Glucosinolates total content, QTL

CLC Number:

S635.03

TIAN Duo-cheng, HE Hong-ju, YAN Hui-ling, XIE Jian-ming, KANG Jun-gen. Mapping QTL and Analysis on Glucosinolates Total Content in Cabbage[J]. journal1, 2014, 29(3): 6-10. doi: 10.7668/hbnxb.2014.03.002.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2014.03.002

http://www.hbnxb.net/EN/Y2014/V29/I3/6

References

[1] 农业部.2006年全国各地蔬菜播种面积和产量[J].中国蔬菜,2008(1):65-66.
[2] 李雷,邹翔,季宇彬.十字花科植物中异硫氰酸盐的性质及活性研究[J].哈尔滨商业大学学报:自然科学版,2007,23(4):385-389,399.
[3] Fahey J W,Zalcmann A T,Talalay P.The chemical diversity and distribution of glucosinolates and isothiocyanates among plants[J].Phytochemistry,2001,56(1):5-51.
[4] Kushad M M,Brown A F,Kurilich A C,et al.Variation of glucosinolates in vegetable crops of Brassica oleracea[J].J Agric Food Chem,1999,47(4):1541-1548.
[5] Rosa E,Heaney K R,Fenwick R,et al.Glucosinolates in cropplants[J].Hort Rev,1997,19:99-215.
[6] 孙秀波,幕美财,李玫瑰,等.十字花科蔬菜硫代葡萄糖苷含量比较[J].安徽农学通报,2007,13(19):64-65.
[7] Cohen J H,Kristal A R,Stanford J L.Fruit and vegetable in takes and prostate cancer risk[J].J Nat l Cancer Inst,2000,92:61-68.
[8] Keck A S,Finley J W.Cruciferous vegetables:cancer protective mechanisms of glucosinolate hydrolys is products and selenium[J].Integr Cancer Ther,2004,3:5-12.
[9] Stoewsand G S,Anderson J L,Munson L.Protective effect of dietary brussels sprouts against mammary carcinogenes is in Sprague-Daw leyrats[J].Cancer Lett,1988,39:199-207.
[10] Wang L G,Liu D L,Ahmed T,et al.Targeting cell cycle machinery as a molecular mechanism of sulforaphane in prostate cancer prevention[J].Internat J On co l,2004,24:187-192.
[11] Wattenberg L W.Inhibition of carcinogenic effects of polycyclic hydrocarbons by benzyl isothiocyanate and related compounds[J].J Nat l Cancer Inst,1977,58(2):395-398.
[12] 何洪巨,陈杭,Schnitle W H.芸薹属蔬菜中硫代葡萄糖苷鉴定与含量分析[J].中国农业科学,2002,35(2):192-197.
[13] 李鲜,陈昆松,张明方,等.十字花科植物中硫代葡萄糖苷的研究进展[J].园艺学报,2006,33(3):675-679.
[14] Gao Muqiang,Li Genyi,Yang Bo,et al.Carlos guiros high-density Brassica oleracea linkage map:identification of useful new linkages[J].Theor Appl Genet,2007 115(2):277-287.
[15] 李占省,刘玉梅,方智远,等.青花菜DH群体花球中莱菔硫烷含量的遗传效应分析[J].园艺学报,2012,39(1):101-108.
[16] 陈健美,管荣展.播娘蒿种子中几种硫苷的提取及鉴定[J].西北植物学报,2006,26(6):1231-1235.
[17] 王丽红,吴莉莉,武冬梅,等.HPLC内标法测定猫耳朵菜的硫苷含量[J].北方园艺,2012(12):128-130.
[18] 朱洪运,颉建明,郁继华,等.结球甘蓝AFLP、SSR、SRAP标记高密度遗传图谱构建[J].华北农学报,2013,28(3):82-87.
[19] McCouch S R,X Chen,O Panaud,et al.Microsatellite marker development,mapping and applications in rice genetics and breeding[J].Plant Mol Biol,1997,35:89-99.
[20] 程坤,仪登霞,杨丽梅,等.甘蓝硫代葡萄糖苷含量与其相关基因表达关系[J].园艺学报,2011,38(增刊):2547.
[21] 陈丽静,王利,王玉坤,等.利用重组自交系群体构建番茄AFLP遗传连锁图谱[J].园艺学报,2012,39(12):2377-2384.
[22] 郝晓芬,王志民,王根全,等.SSR方法标记谷子光敏雄性不育基因[J].华北农学报,2011,26(5):112-116.
[23] 于栓仓,王永健,郑晓鹰.大白菜分子遗传图谱构建[J].中国农业科学,2003,36(2):190-195.

Please choose a citation manager

Content to export