QTL Analysis on Stomatal Trait of Leaf in Rice

doi:10.7668/hbnxb.2014.03.001

Abstract

Abstract: Using backcross recombinant inbred lines derived from a cross between a tropical japonica, Sasanishiki and an indica variety, Habataki, quantitative trait loci(QTLs) controlling the stoma related traits of flag leaf by 236 RFLP molecular markers from genetic linkage map in rice were analyzed.A total of four QTLs for stoma related traits were identified.Three putative QTLs (qSD2, qSD4 and qSD12) for stomatal density were mapped on chromosome 2, 4 and 12 with the contribution of each QTL was 13.11%, 11.76% and 22.70%, respectively.On chromosome 3 of rice, one QTL(qSW3)for stomatal width was detected, with the explained variance of 10.04%.The alleles for stomatal density and stomatal width were contributed by Sasanishiki and Habataki, respectively.Significant negative differences were found between stomatal density and stomatal length(r=-0.509A), while significant positive correlations were observed between stomatal length and stomatal width(r=0.302A).All the three QTLs controlling stomatal density were not previously reported, but qSW3 controlling stomatal width was overlapped with qTLN-3a controlling total nitrogen content from other study.It reflected the genetic complexity of stomatal traits.These results will help to reveal the genetic mechanism of stomatal trait in rice and provide vital reference for the molecular mechanism of difference of stomatal traits between indica and japonica rice.

Key words: Rice, Flag leaf, Stomatal trait, Quantitative trait loci

CLC Number:

S511.03

GUO Zhi-fu, LI Xiao-lin, ZHAO Ming-hui, MA Dian-rong, MA Hui, XU Hai, XU Zheng-jin. QTL Analysis on Stomatal Trait of Leaf in Rice[J]. journal1, 2014, 29(3): 1-5. doi: 10.7668/hbnxb.2014.03.001.

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