Cloning of ovine Prolactin Releasing Hormone and Its Receptor Gene and Their Different Expression in the HPG Axis

doi:10.7668/hbnxb.2015.03.004

Abstract

Abstract: To explore the expression of PRLH and its receptor gene in sheep gonadal axis,we successfully cloned the PRLH and receptor gene in organizations at the hypothalamic-pituitary-gonadal axis by used the sheep as experimental animal,got the phylogenetic tree.Also we used qRT-PCR technology to research different expression of PRLH and its receptor gene in different tissues at HPG axis.The results showed that sheep PRLH and PRLHR nucleotide sequences of mRNA were 113,237 bp.PRLH gene nucleotide sequence and goats,cattle,human,mouse homology were 96.8%,93.3%,80.3%,74.6%.PRLHR gene and the Tibetan antelope,goat,cattle,human and mouse homology were 100%,99.3%,97.3%,90.4%,84.4%.PRLH and its receptor gene in sheep hypothalamus,pituitary,uterine and ovary were expressed,and the expression of PRLH gene in hypothalamus,pituitary and ovary was significantly less than the uterine (P<0.05).Expression of PRLHR in uterus and pituitary were significantly different (P<0.05),no significant difference in other tissues (P>0.05).So the hypothalamus,pituitary,ovary and uterus were the main organ for the synthesis of secretion.

Key words: Sheep, PRLH, PRLHR, RT-PCR, Differential expression

CLC Number:

ZHAO Qiang, MA You-ji, LI Jing-hua. Cloning of ovine Prolactin Releasing Hormone and Its Receptor Gene and Their Different Expression in the HPG Axis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(3): 20-24. doi: 10.7668/hbnxb.2015.03.004.

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