Transcriptome Analysis on Transcription Factors of Haloxylon ammodendron under Drought Stress and Rehydration Treatment

doi:10.7668/hbnxb.20190513

Abstract

Abstract: In order to elucidate the molecular regulation mechanism of H.ammodendron in response to drought stress and water stimulation,the transcriptome and expression profile of transcription factors of H.ammodendron in normal watering group(CK),drought stress groups(light water stress group (LWS),moderate water stress group(MWS),severe water stress group(SWS))and rehydration group after drought(RSWS)were systematically analyzed. The results showed that 74 641 non-redundancy unigenes with an average N50 length of 1 537 bp were obtained after being de novo assembled. And 1 307 transcription factor encoding genes belonging to 56 families were finally screened out by comparing and annotating the transcriptome data. Compared with the control group,the differentially expressed genes in the four treatment groups were mainly down-regulated,and the expression of AP2-EREBP,MYB,bHLH,WRKY,NAC,and ABI3VP1 transcription factors family were the most significant. In the rehydration group(RSWS vs SWS),the response to water stimulation of H.ammodendron seedlings was regulated by increasing the number of up-regulated AP2-EREBP,WRKY and NAC coding genes,however,in the drought-stress group(CK vs LWS,C vs MWS,CK vs SWS),the coding genes number of AP2-EREBP and bHLH was mainly down regulated. Nine transcription factor coding genes were randomly selected from the sequencing data. Real-time PCR was used to verify the validity of the sequencing data. The results showed that the correlation between the expression of FPKM in drought and rehydration groups and the gene expression obtained by fluorescence quantitative PCR was 0.947 8(P<0.01)and 0.967 2(P<0.01),respectively. In conclusion,the family members of AP2-EREBP,MYB and bHLH plays positive and negative regulation effect in response to the drought stress of H.ammodendron.

Key words: Haloxylon ammodendron, Transcription factor, Transcriptome, Drought stress, Differential expression

CLC Number:

S793.9

HE Jiangfeng, WANG Liwei, FANG Yongyu, WANG Yunhua, WANG Chao, LIU Hongkui. Transcriptome Analysis on Transcription Factors of Haloxylon ammodendron under Drought Stress and Rehydration Treatment[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(1): 36-43. doi: 10.7668/hbnxb.20190513.

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