Polypeptide Analysis of GIFT Tilapia Liver after Infecting Streptococcus agalactiae

doi:10.7668/hbnxb.2016.S1.083

Abstract

Abstract: The polypeptide expression on GIFT tilapia liver was studied after infecting Streptococcus agalactiae.Matrix-assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) was employed to analyze the polypeptide,and they were identified by mass spectrometry.The results indicated that there were 44 and 52 polypeptide between control group and experimental group,which Signal/Noise (s/n) was more than 50.We discovered 6 peaks (m/z 1 082, 1 161,1 196,1 459,1 740,1 877) was obvious difference in two groups(P<0.01).The 1 196(m/z) peptide decreased,but the 1 161 and 1 459(m/z) peptide increased in experimental group.All the mass spectrums were corresponding to 19 peptides,and 14 shows a up-regulated expression,4 shows down-regulated expression,and 1 shows no obvious change.The results of bioinformatical analysis indicated that all the peptides were involved in three regulation pathways including TCA cycle,DNA splice and cell defensive signaling pathway.Real-time PCR was utilized to analysis the mRNA variation of ATP synthase 6 (ATP6) gene and D-beta-hydroxybutyrate dehydrogenase (Bhd1) gene.The results confirmed the positive correlation of gene expression and protein expression.The results of peptidome analysis provided a new approach to diagnose the Streptococcus agalactiae disease.

Key words: GIFT tilapia, Streptococcus agalactiae, Polypeptide, Differential expression

CLC Number:

ZHU Jiajie, FU Qiang, AO Qiuwei, CHEN Ming, TAN Yun, LI Liping, JIANG Hesheng, GAN Xi. Polypeptide Analysis of GIFT Tilapia Liver after Infecting Streptococcus agalactiae[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(S1): 485-489. doi: 10.7668/hbnxb.2016.S1.083.

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