Identification and Sequence Analysis of SRAP Markers Linked to Salt-tolerance Gene in Apple Rootstock

doi:10.7668/hbnxb.2015.02.012

Abstract

Abstract: The study was conducted to screen molecular markers of salt-tolerance gene in apple rootstock, which providing a theoretical basis for molecular assistant selection breeding in plants salt-tolerance.F₁ of Malus micromalus ×S19 were used to screen markers linked to salt-tolerance gene by BSA method and SRAP technology.Four pairs of primers (Me1Em2, Me1Em8, Me6Em12 and Me6Em14) were screened from 128 ones, which expressed polymorphism in parents and DNA pools and totally produced 4 polymorphic fragments.The four pairs of primers were tested by 144 F₁ hybrid seedings, and the coincidence rate between hydroponic screening and the SRAP analysis were between 81.94% and 92.36%.The length of the 4 polymorphic fragments (Ⅰ, Ⅱ, Ⅲ and Ⅳ)was between 139 bp and 233 bp by cloning and sequencing.Sequence alignment revealed that sequenceⅠhad relatively highly identity with ATP synthase beta subunit gene(ATP-β) from Photinia fraseri, and there was an identity of 98% for nucleotide sequence.Additionally, other sequences showed partial identity with probable receptor-like protein kinase from Pyrus × Bretschneideri, myosin from Malus domestica, and UDP-glycosyltransferase from Malus domestica, respectively.The 4 markers selected not only could be used in molecular identification of salt tolerance, but also provide a foundation to clone salt-tolerance gene for apple rootstock.

Key words: Salt-tolerance, SRAP, Differential expression analysis

CLC Number:

Q78
S661.03

SUN Ye-hong, ZHANG Yuan, LI Zhong-yong, SHAO Jian-zhu, XU Ji-zhong. Identification and Sequence Analysis of SRAP Markers Linked to Salt-tolerance Gene in Apple Rootstock[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(2): 59-63. doi: 10.7668/hbnxb.2015.02.012.

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