Mitochondrial DNA Difference Analysis between CMS and Its Maintainer Line of Pepper through SRAP

doi:10.3969/j.issn.1000-7091.2012.06.014

Abstract

Abstract: SRAP ( Sequence-related amplified polymorphism) was analyzed between mitochondrial DNA of cytoplasm male sterility line 21A and its maintainer line 21B of pepper． The analysis showed that a total of 1 440 amplified fragments and nine polymorphic fragments between 100-1 000 bp were detected by applying 128 SRAP primer combinations; the polymorphic fragment percentage was 0． 63%; seven of the polymorphic fragments was detected in male sterility line 21A． The polymorphic fragments were isolated，cloned and sequenced，blast of the 9 clones in GenBank showed that it all have homologous with energy metabolism genes． According to the sequences， three specific primers were designed to transform the SRAP marker to more stable SCAR markers，and they only detected in the 21A line， this suggested that the newly detected fragments related to cytoplasm male sterility．

Key words: Pepper, CMS, Mitochondrial DNA, SRAP

CLC Number:

S641.03

YUAN Wen, WU Guo-ping, WANG Shu-bin, DIAO Wei-ping, LIU Jin-bing, PAN Bao-gui, GE Wei. Mitochondrial DNA Difference Analysis between CMS and Its Maintainer Line of Pepper through SRAP[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 62-66. doi: 10.3969/j.issn.1000-7091.2012.06.014.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3969/j.issn.1000-7091.2012.06.014

http://www.hbnxb.net/EN/Y2012/V27/I6/62

References

[1] 王述彬，罗向东，戴亮芳，等．辣椒细胞质雄性不育系与其保持系线粒体DNA 的RAPD 分析[J]．江苏农业学报， 2008， 24( 1) : 44-47．
[2] Jo Y D， Jeong H J，Kang B C． Development of a CMS-specific marker based on chloroplast-derived mitochondrial sequence in pepper[J]． Plant Biotechnol Rep，2009，3: 309-315．
[3] 刘科伟，王述彬，刘金兵，等．辣椒细胞质雄性不育基因的AFLP 分析[J]．分子植物育种， 2009，7( 4) : 736-742．
[4] Kim D H，Kang J G，Kim B D． Isolation and characterization of the cytoplasmic male sterility associated orf456 gene of chili pepper ( Capsicum annuum L．) [J]． Plant Mol Biol， 2007， 63: 519-532．
[5] 王永飞，马三梅，张鲁刚，等．大白菜细胞质雄性不育系和其保持系的RAPD 分析[J]．西北植物学报，2003， 23( 4) : 554-560．
[6] 邓晓辉，张蜀宁，侯喜林．白菜Pol 胞质雄性不育系和保持系蕾期基因表达差异的SRAP 分析[J]．园艺学报， 2007， 34( 3) : 655-658．
[7] 冒维维，高红胜，薄天岳，等．菜薹雄性不育相关基因的ISSR 分子标记筛选[J]．分子植物育种，2009，7( 1) : 40-44．
[8] 姚雪琴，李媛，谢祝捷，等．青花菜胞质不育相关基因的SRAP 标记筛选[J]．分子植物育种，2009，5: 941-947．
[9] 李亚利，扈新民，赵丹，等．运用SRAP 分子标记鉴定辣椒杂交种纯度[J]．中国农学通报，2010，26 ( 24) :67-70．
[10] 张素勤，耿广东，周贤婷，等．贵州辣椒种质资源的表型和SRAP 分析[J]．山地农业生物学报，2008，27( 3) : 228-232．
[11] 许先松，刘志钦，林晓丹，等．基于形态及SRAP 标记的辣椒资源遗传多态性及亲缘关系比较[J]．福建农林大学学报: 自然科学版， 2011， 40( 1) : 48-53．
[12] 陈学军，方荣，周坤华，等．辣椒中间杂种的表型鉴定及SRAP 分析[J]．西北植物学报，2011，31 ( 2) :286-290．
[13] 任羽，张银东，尹俊梅，等．应用SRAP 分子标记评价辣椒自交系的遗传关系[J]．热带作物学报， 2008，29( 1) : 28-52．
[14] Kohler R H，Horn R，Lossl A，et al． Cytoplasmic male sterility in sunflower is correlated with the co-transcription of a new open reading frame with the atpA gene
[J]． Mol Gen Genet， 1991， 227( 3) : 369-376．
[15] Akagi H，Sakamoto M，Shinjyo C，et al． A unique sequence located downstream from the rice mitochondrial atp6 may cause male sterility[J]． Theor Appl Genet， 1994， 25: 52-58．
[16] Singh M，Brown G G． Suppression of cytoplasmic male sterility by nuclear genes alters expression of a novel mitochondrial gene region[J]． The Plant Cell，1991，3 ( 12) : 1349-1362．
[17] Singh M，Brown G G． Characterization of expression of a mitochondrial gene region associated with the brassica " polima" CMS developmental influences[J]． Curr Genet， 1993， 24( 4) : 316-322．
[18] Kemble R J． A rapid， single leaf，nucleic acid assay for determining the cytoplasmic organelle complement of rapeseed and related Brassica species[J]． Theor Appl Genet， 1987， 73: 364-370．
[19] Li G，Quiros C F． Sequence-related amplified polymorphism( SRAP),a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica．[J]． Theor Appl Genet， 2001， 103: 455-461．
[20] Folkerts O Hanson M R． Three copies of a single recombinization repeat occur on the443kb master circle of the petunia hybrida 3704 mitochondrial DNA genome[J]． Nucleic Acids Res， 1989， 17: 7345-7357．
[21] Dewey R E，Levings C S，Timothy D H． Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm[J]． Cell， 1986， 44: 439-449．
[22] Young E G，Hanson M R，Dierks P M． Sequence and transcription analysis of the Petunia mitochondrial gene for the ATP synthesis proteolipid subunit[J]． Nucleic Acids Rea， 1986， 14: 7995-8006．
[23] Bonhomme S，Budar F，Lancelin D， et al． Sequence and transcript analysis of the Nco2． 5 Ogura-specific fragment correlated with cytoplasmic male sterility in Brassica cybrids[J]． Mol Gel Genet， 1992， 235: 340-348．
[24] Mackenzie S，Mclntosh L． Higher plant mitochondria[J]． Plant Cell， 1999， 11: 571-585．
[25] L'Homme Y，Brown G G． Organizational differences between cytoplasmic male sterile and male fertile Brassica mitochondrial genomes are confined to a single transposed locus[J]． Nucleic Acids Research， 1993， 21( 8) : 1903-1909．
[26] Brown G G，Domaj M，Du Pauw M， et al． Molecular analysis of Brassica CMS and its application to hybrid seed production[J]． Acta Horticulturae，1998，459: 265-274．
[27] Yang J H，Zhang M F，Yu J Q． Mitochondrial nad2 gene is co-transcripted with CMS-associated orfB gene in cytoplasmic male-sterile stem mustard ( Brassica juncea )[J]． Mol Bio Rep， 2009， 36( 2) : 345-351．

Please choose a citation manager

Content to export