Effects of Different Nuclear Backgrounds on Mitochondrial Complex Activity of CMS-C in Maize

doi:10.7668/hbnxb.20192358

Abstract

Abstract: In order to explore the influence of mitochondrial complex activity on the fertility of C-type male sterile lines in maize under different nuclear backgrounds, and to clarify its relationship with anther abortion. Plant mitochondrial respiratory chain complex enzyme linked immunoassay kit was used to determine the activities of 5 mitochondrial complex in different stages of anther development, using homogeneous heterogenic male sterile lines K932S and K169S, restorer line K932R and maintainer line K169 as materials. Results showed that in addition to the period of pollen mother cell complex Ⅱ, Ⅳ, the remaining period of sterile cytoplasm complex Ⅰ, Ⅱ, Ⅳ activity under the background of K169 nuclear was significantly higher than K932, most of the time the activity of complex Ⅲ, Ⅴ, on the other hand, when there was a restoring genes but K932R might change the part of the activity of sterile cytoplasm during different mitochondrial complex. The activity of sterile cytoplasmic complexes Ⅱ, Ⅲ and Ⅴ was significantly lower than that of normal fertile cytoplasm at the diad stage, while the activity of five complexes at most other stages was the opposite. It can be seen that the nuclear background have a significant effect on the activity of different mitochondrial complexes of CMS-C in maize at different anther development stages. Under the same nuclear background, the activity of cytoplasmic complexes Ⅱ, Ⅲ and V at the diploid stage was significantly lower than that of normal cytoplasm, which might be closely related to CMS-C drug abortion.

Key words: Maize, CMS-C, Mitochondrial complex

CLC Number:

S513.01

ZHAO Changyun, ZHANG Qiming, SHI Haichun, YU Xuejie, DONG Li, XIA Wei, KE Yongpei. Effects of Different Nuclear Backgrounds on Mitochondrial Complex Activity of CMS-C in Maize[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 89-93. doi: 10.7668/hbnxb.20192358.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20192358

http://www.hbnxb.net/EN/Y2021/V36/IS1/89

References

[1] Henze K,Martin W. Essence of mitochondria[J]. Nature,2003,426(6963):127-128.doi:10.1038/426127a.
[2] 郝岗平,陈敏,杨清. 植物线粒体与细胞质雄性不育研究进展[J].植物学通报,2003,20(5):549-557.doi:10.3969/j.issn.1674-3466.2003.05.005.
Hao G P,Chen M,Yang Q. Progress of study on plant mitochondria and cytoplasmic male sterility[J]. Chinese Bulletin of Botany,2003,20(5):549-557.
[3] Budar F,Touzet P,De Paepe R. The nucleo-mitochondrial conflict in cytoplasmic male sterilities revisited[J]. Genetica,2003,117(1):3-16.doi:10.1023/A:1022381016145.
[4] 周琼,周瑶瑶,唐向民,周瑞阳,李春瑶. 红麻细胞质雄性不育系与保持系花药活性氧代谢差异比较[J].广西植物,2013,33(4):443-448.doi:10.3969/j.issn.1000-3142.2013.04.003.
Zhou Q,Zhou Y Y,Tang X M,Zhou R Y,Li C Y. Comparative research of reactive oxygen species metablisms of the CMS line and the maintainer lines in Kenaf(Hibiscus cannabinus)anther[J]. Guihaia,2013,33(4):443-448.
[5] 周凯,司龙亭,张琪. 萝卜细胞质雄性不育系与保持系光合特性和呼吸特性的比较试验[J].北方园艺,2007(6):3-5.doi:10.3969/j.issn.1001-0009.2007.06.002.
Zhou K,Si L T,Zhang Q. The comparative research of the photosynthesis and the respiration of the CMS lines and the maintainer lines of radish[J]. Northern Horticulture,2007(6):3-5.
[6] 张明永,梁承邺,黄毓文,刘鸿先.水稻细胞质雄性不育系与保持系的呼吸途径比较[J].植物生理学报,1998,24(1):55-58.
Zhang M Y,Liang C Y,Huang Y W,Liu H X. Comparison of respiratory pathways of CMS and its maintainer rice(Oryza sativa L.)[J]. Plant Physiology Journal,1998,24(1):55-58.
[7] 邓明华,文锦芬,邹学校,何长征,刘志敏,周群初. 辣椒核质互作雄性不育系与保持系呼吸速率研究[J].云南农业大学学报,2009,24(1):22-25.doi:10.3969/j.issn.1004-390X.2009.01.005.
Deng M H,Wen J F,Zou X X,He C Z,Liu Z M,Zhou Q C. A study on respiration rates in cytoplasmic male-sterile lines and their maintainer lines of Capsicum annuum L[J]. Journal of Yunnan Agricultural University,2009,24(1):22-25.
[8] 严志祥. 紫稻细胞质雄性不育系水稻线粒体呼吸链初步研究[D].武汉:武汉大学,2009.
Yan Z X. Study on mitochondrial respiratory chain of zidao type cytoplasmic male sterility rice(Oryza sativa L.)[D].Wuhan:Wuhan University,2009.
[9] Walker J E,Arizmendi J M,Dupuis A,Fearnley I M,Finel M,Medd S M,Pilkington S J,Runswick M J,Skehel J M.Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria:Application of a novel strategy for sequencing proteins using the polymerase chain reaction[J]. Journal of Molecular Biology,1992,226(4):1051-1072.doi:10.1016/0022-2836(92)91052-Q.
[10] Schnable P S,Wise R P.The molecular basis of cytoplasmic male sterility and fertility restoration[J]. Trends in Plant Science,1998,3(5):175-180.doi:10.1016/S1360-1385(98)01235-7.
[11] Watson C V,Nath J,Nanda D. Possible mitochondrial involvement in mechanism of cytoplasmic male sterility in maize(Zea mays L.)[J]. Biochemical Genetics,1977,15(11/12):1113-1124.doi:10.1007/BF00484501.
[12] 孔祥军. 海岛棉H276A雄性不育的细胞学与分子生物学基础研究[D].南宁:广西大学,2017.doi:10766/d.Y3352141.
Kong X J. Studies on cytology and molecular biology of male sterility line H276A in Gossypium barbadense[D].Nanning:Guangxi University,2017.
[13] 刁勇.红麻UG93细胞质雄性不育相关基因的筛选、克隆及表达分析[D].南宁:广西大学,2016.doi:10.7666/d.Y3088037.
Diao Y.Screening,cloning and expression analysis of mitochondrial genes related to CMS UG93 in kenaf[D].Nanning:Guangxi University,2017.
[14] 向杰. 玉米C型细胞质雄性不育的线粒体差异蛋白质分析[D].雅安:四川农业大学,2010.
Xiang J. Analysis of differential mitochondrial proteins from CMS-C maize[D].Yaan:Sichuan Agricultural University,2010.
[15] 周国昌. 玉米雄性不育系K932S的遗传分析[D].雅安:四川农业大学,2018.
Zhou G C. Genetic Analysis of male sterility line K932S in maize[D].Yaan:Sichuan Agricultural University,2018.
[16] Sheoran I S,Sawhney V K.Proteome analysis of the normal and Ogura(ogu)CMS anthers of Brassica napus to identify proteins associated with male sterility[J]. Botany,2010,88(3):217-230.
dio:10.1139/B09-085.
[17] 黄晋玲. 棉花晋A细胞质雄性不育系的遗传研究[D].太谷:山西农业大学,2003.
Huang J L. Genetie study on Jin A cytoplasmic male sterile line in cotton(Gossypium)[D].Taigu:Shanxi Agricultural University,2003.
[18] 袁建玉. 不结球白菜CMS新种质细胞学及生理生化特性研究[D].南京:南京农业大学,2005.doi:10.7666/d.Y749126.
Yuan J Y. Study on cytomorphology and characteristies of physiology and biochemistry in the new CMS line of non-heading Chinese cabbage[D].Nanjing:Nanjing Agricultural University,2005.
[19] 杜坤. 甘蓝型油菜细胞质雄性不育系SaNa-1A败育的细胞学和分子基础[D].扬州:扬州大学,2016.
Du K. Cytology and molecular analysis on sterile mechanism of SaNa-1A CMS in Brassica napus[J].Yangzhou:Yangzhou University,2016.
[20] 王秀珍,滕晓月,阎隆飞,周人纲. 玉米及高粱花药中三磷酸腺苷(ATP)含量与细胞质雄性不育的关系[J].作物学报,1986,12(3):177-181.doi:10.3321/j.issn:0496-3490.1986.03.005.
Wang X Z,Teng X Y,Yan L F. The relationship between the ATP content in anthers of maize and sorghum and cytoplasmic male-sterility[J]. Acta Agronomica Sinica,1986,12(3):177-181.

Please choose a citation manager

Content to export