Efficiency Comparative Analysis of Three Different Nucleic Acid Analysis System

doi:10.3969/j.issn.1000-7091.2012.06.013

Abstract

Abstract: Four kinds of genetically modified maize genomic DNA were extracted，using specific primers for PCR and multiplex PCR detection in this study． The PCR products were analyzed respectively by ordinary agarose gel electrophoresis and other three kinds of nucleic acid analyzer ABI 3730XL，Shimadzu MultiNA and QIAGEN QIAxcel． The experimental results were compared and summarized the advantages and disadvantages of the detection methods for nucleic acid fragments of different size，which provided an important reference for different analysis requirements．

Key words: Transgenic detection, Multiplex PCR, Nucleic acid analysis system

CLC Number:

LIU Ya, LI Xiang, REN Wen, JIANG Hui-quan, ZHAO Jiu-ran. Efficiency Comparative Analysis of Three Different Nucleic Acid Analysis System[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(6): 58-61. doi: 10.3969/j.issn.1000-7091.2012.06.013.

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