Abstract: High-molecular-weight glutenin subunits(HMW-GS) are closely correlated with the processing quality of common wheat.Establishment related select systems which are simple and convenient is very important to evaluate wheat quality and select new wheat cultivars with good qualities.According to the molecular markers for genes controlling important qualities,four types of multiplex PCR detection systems for strong-gluten wheat were developed and validated using 26 Chinese wheat cultivars and advanced lines with known HMW-GS(genes).The first multiplex PCR was used to simultaneously detect genes Axnull and By8,the second one was to detect the genes By8 and Dx5,the third multiplex PCR included three genes Axnull,By8 and Dx5,the fourth one was to detect the genes Axnull,Bx14 and Dx5.After the 26 Chinese wheat cultivars were detected using the four types of multiplex PCRs,respectively,their results were consistent with those of the known.The results indicated that the four multiplex PCRs established in the present study were stable and reliable.The multiplex PCRs would be effectively used to evaluate and select wheat quality properties in wheat breeding programs.

Key words: Wheat, High-molecular-weight glutenin subunit, Multiplex PCR

CLC Number: 

• S512．03