Genetic Diversity of Sclerotinia sclerotiorum Resistant Brassica napus Based on SSR and SRAP

doi:10.7668/hbnxb.2016.03.025

Abstract

Abstract: In order to disclose the genetic diversity of Brassica napus accurately,explore the application of two molecular markers(SSR and SRAP )in genetic diversity analysis in Sclerotinia sclerotiorum resistant Brassica napus, forty core SSR primers and forty selected SRAP primers with high polymorphism and clear bands from molecular and genetic laboratory in department of Biological of Shaanxi University of Technology were used to analyze 43 Sclerotinia sclerotiorum resistant rapeseed materials identified with stem inoculation method by Hanzhong Institute of Agricultural Sciences.The polymorphism bands and PIC value revealed by the two markers were analyzed.The results showed that,634 polymorphism bands were detected by two markers,the polymorphism bands detected by SRAP(335)was higher than SSR(287),but the average PIC value of SSR primer was higher than SRAP primer,that was 0.76 and 0.69 respectively.The 43 rapeseed accessions were divided into three clusters at the coefficient cut off value 0.67.The principal component analysis(PCA)and the population structure analysis results were similar to the result of cluster analysis,showed that combination of SSR and SRAP marker could reflect the relationship of rapeseed accurately.Genetic similarity coefficient of the 43 rapeseed samples distributed mainly between 0.65 and 0.81,displayed that the tested rapeseed materials had a near relationship and a relatively high genetic similarity.Thus,it's of great importance to introduce and enhance the selection of Sclerotinia sclerotiorum resistant lines to broaden the genetic background of current ones for further Sclerotinia sclerotiorum resistance breeding.

Key words: SSR, SRAP, Brassica napus, Sclerotinia sclerotiorum, Genetic diversity

CLC Number:

S565.03

ZHANG Xiaojuan, ZHANG Yu, ZHAO Hui. Genetic Diversity of Sclerotinia sclerotiorum Resistant Brassica napus Based on SSR and SRAP[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 169-174. doi: 10.7668/hbnxb.2016.03.025.

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