Effect of Castration on PPAR Signal Pathway Expression in Huainan Pig Subcutaneous Fat Tissue

doi:10.7668/hbnxb.2016.02.016

Abstract

Abstract: To detect the role of PPAR signal pathway on the castration-induced fat deposition, RNA was isolated from the subcutaneous fat tissues of three castrated Huainan pigs and three intact Huainan pigs.The high-throughput sequencing approach was used to identify mRNA expression difference in castrated and intact pigs.The screening threshold for these genes was P value < 0.05 and|log₂ FoldChange|> 1.Based on the difference of the mRNA and the KEGG enrichment analysis, the expression of PPAR signal pathway was the highest in the castrated pigs, of which fourteen genes were upregulated and three genes were downregulated.Lipogenic genes like fatty acid binding protein 7 gene(FABP7), aquaporin 7 gene(AQP7), retinoid X receptor, gamma gene(RXRγ) were upregulated significantly in castrated pigs, and lipolysis genes were downregulated like phosphoenolpyruvate carboxykinase 1 gene(PCK1), peroxisome proliferator-activated receptor gamma, coactivator 1 alpha gene(PPARGC1α) and cytochrome P450, family 7, subfamily A, polypeptide 1 gene(CYP7A1).

Key words: Huainan pig, Differential expression gene, High throughput sequencing, PPAR signal pathway, Fat deposition

CLC Number:

S828
Q78

WANG Jing, BAI Xianxiao, ZHANG Jiaqing, GAO Binwen, CHEN Junfeng, GAO Yuan, REN Qiaoling, MA Qiang, GUO Hongxia, LIANG Yonghong, XING Baosong. Effect of Castration on PPAR Signal Pathway Expression in Huainan Pig Subcutaneous Fat Tissue[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(2): 92-97. doi: 10.7668/hbnxb.2016.02.016.

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