Transcriptome Profiling of Loquat Leaves under Drought Stress

doi:10.7668/hbnxb.2017.01.010

Abstract

Abstract: The transcriptome of loquat leaves under drought stress were sequenced by Illumina HiSeqhigh-throughput platform which is a new generation of sequencing technology. This study was conducted to elucidate functional gene, analyzed its differential expressed gene and provided a theoretical basis for its genetic improvement. The sequencing data was assembled by de novo assembly and the differential expressed gene screened was annotated in COG, GO and KEGG database. The sequencing results showed that 88 530 transcripts with the mean length of 740.64 bp were conducted. 41 748 ORFs were predicted by opening reading fragment.Clusters of orthologous groups performed the transcripts into 24 categories.All assembled transcripts and ORFs were divided into 54 branches by Gene ontology and 291 pathways by the Kyoto Encyclopedia of Genes and Genomes annotations analyses.25 197 DEGs were enriched in 30 metabolic pathways and numerous genes related to enzyme activity,hormone metabolism and signal transduction inferred that might be connected with drought stress of loquat.Moreover, DEGs in carotenoid biosynthesis, brassinosteroid biosynthesis and diterpenoid biosynthesis showed a similar expression.Furthermore, DEGs were confirmed by qRT-PCR and peroxidase,protein kinase byr2, Serine/threonine protein kinase,abscisic acid 8'-hydroxylase and indole-3-acetic acid-amido synthetase were down regulated, Cytochrome P450 734A1 was on the contrary.This study not only provided valuable genetic resources and a comprehensive map of drought-responsive genes in this species but also a data basis for further explorer molecular response mechanism to drought stress in loquat.

Key words: Loquat, Drought stress, Transcriptome, Differential expression

CLC Number:

Q78
S667.3

WANG Dan, GONG Ronggao. Transcriptome Profiling of Loquat Leaves under Drought Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(1): 60-67. doi: 10.7668/hbnxb.2017.01.010.

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http://www.hbnxb.net/EN/Y2017/V32/I1/60

References

[1] Carbonell F B, Barrachina A C, Roig A V, et al. Effects of irrigation water quality on loquat plant nutrition:Sensitivity of loquat plant to salinity[J]. Journal of Plant Nutrition, 1997, 20(1):119-130.
[2] 罗华建,刘星辉.水分胁迫条件下枇杷若干生理指标的变化[J].亚热带植物科学, 2004, 33(1):19-21, 25.
[3] Stellfeldt A, Hueso J J, Cuevas J. No need for further fruit thinning in water-deprived loquat trees at preharvest[J]. Scientia Horticulturae, 2013, 162(3):144-149.
[4] Ashraf M. Inducing drought tolerance in plants:recent advances[J]. Biotechnology Advances, 2009, 28(1):169-183.
[5] 杨再强,谢以萍,张旭东,等.水分胁迫对枇杷果实发育阶段的光合特性和果实品质的影响[J].灌溉排水学报, 2007, 6(26):89-92.
[6] Fernández M D, Hueso J J, Cuevas J. Water stress integral for successful modification of flowering dates in Algerie loquat[J]. Irrigation Science, 2010, 28(2):127-134.
[7] Hueso J J, Cuevas J. Loquat as a crop model for successful deficit irrigation[J]. Irrigation Science, 2008, 26(3):269-276.
[8] Yang Y, Xu M, Luo Q, et al. De novo transcriptome analysis of Liriodendron chinense petals and leaves by Illumina sequencing[J]. Gene, 2013, 534(2):155-162.
[9] 贾新平,孙晓波,邓衍明,等.鸟巢蕨转录组高通量测序及分析[J].园艺学报, 2014, 41(11):2329-2341.
[10] Seki M, Narusaka M, Ishida J, et al. Monitoring the expression profiles of 7000Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray[J]. The Plant Journal:for Cell and Molecular Biology, 2002, 31(3):279-292.
[11] Rabbani M, Maruyama K, Abe H, et al. Monitoring expression profiles of rice genes under cold, drought, and high-salinity stresses and abscisic acid application using cDNA microarray and RNA gel-blot analyses[J]. Plant Physiology, 2003, 133(4):1755-1767.
[12] Priest H D, Fox S E, Rowley E R, et al. Analysis of global gene expression in Brachypodium distachyon reveals extensive network plasticity in response to abiotic stress[J]. PLoS One, 2014, 9(1):e87499.
[13] 王洁.干旱胁迫马铃薯叶片转录组分析[D].兰州:甘肃农业大学, 2014.
[14] Grabherr M G, Haas B J, Yassour M, et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome[J]. Nature Biotechnology, 2011, 29(7):644-652.
[15] Hayano-Kanashiro C, Calderón-Vázquez C, Ibarra-Laclette E, et al. Analysis of gene expression and physiological responses in three Mexican maize landraces under drought stress and recovery irrigation[J]. PLoS One, 2009, 4(10):69.
[16] 宋杨,刘红弟,张红军,等.越橘果实转录组及R2R3-MYB转录因子分析[J].园艺学报, 2015, 42(12):2383-2394.
[17] 费元,韩雪,余红,等.大岩桐花萼和幼叶转录组研究[J].园艺学报, 2015, 42(12):2519-2525.
[18] 麻文俊.楸树优良无性系2-8苗期生理变化与基因表达对干旱胁迫的响应[D].北京:中国林业科学研究院, 2013.
[19] Wu Y, Wei W, Pang X, et al. Comparative transcriptome profiling of a desert evergreen shrub, Ammopiptanthusmongolicus, in response to drought and cold stresses[J]. BMC Genomics, 2014,15(1):1-16.
[20] Lata C, Sahu P P, Prasad M. Comparative transcriptome analysis of differentially expressed genes in foxtail millet (Setaria italica L.) during dehydration stress. Biochemical and Biophysical Research Communications[J],2010,393(4):720-727.
[21] 蔡国华.玉米促分裂原活化蛋白激酶激酶基因ZmMKK1的分离及功能分析[D].泰安:山东农业大学, 2014.
[22] Li J, Tax F. Receptor-like kinases:key regulators of plant development and defense[J]. Journal of Integrative Plant Biology, 2013, 55(12):1184-1187.
[23] Hassan N, El-Bastawisy Z, El-Sayed A K, et al. Roles of dehydrin genes in wheat tolerance to drought stress[J]. Journal of Advanced Research, 2013, 6(2):179-188.
[24] Zhang G, Chen M, Li L, et al. Overexpression of the soybean GmERF3 gene, an AP2/ERF type transcription factor for increased tolerances to salt, drought, and diseases in transgenic tobacco[J]. Journal of Experimental Botany,2008,60(13):3781-3796.
[25] Li J S, Fu F L, An M, et al. Differential expression of MicroRNAs in response to drought stress in maize[J]. Journal of Integrative Agriculture, 2013, 12(8):1414-1422.
[26] Kumar V, Malik S K, Pal D, et al. Comparative transcriptome analysis of ovules reveals stress related genes associated with nucellar polyembryony in citrus[J]. Tree Genetics & Genomes, 2014, 10(3):449-464.
[27] Chen L, Song Y, Li S, et al. The role of WRKY transcription factors in plant abiotic stresses[J]. Biochimica et Biophysica acta, 2012, 1819(2):120-128.
[28] 巩檑,张丽,聂峰杰,等.干旱胁迫和复水处理后马铃薯转录因子的转录组分析[J].分子植物育种, 2015, 13(8):1745-1756.
[29] Nambara E, Marion-Poll A. Abscisic acid biosynthesis and catabolism[J]. Annual Review of Plant Biology, 2005,56(56):165-185.
[30] 黄彬城,季静,王罡,等.植物类胡萝卜素的研究进展[J].天津农业科学, 2006, 12(2):13-17.
[31] Umezawa T, Okamoto M, Kushiro T, et al. CYP707A3, a major ABA 8'-hydroxylase involved in dehydration and rehydration response in Arabidopsis thaliana[J].Plant Journal,2006, 46(2):171-182.
[32] Okamoto M, Kuwahara A, Seo M, et al. CYP707A1 and CYP707A2, which encode abscisic acid 8'-hydroxylases, are indispensable for proper control of seed dormancy and germination in Arabidopsis[J]. Plant Physiology, 2006, 141(1):97-107.
[33] 裴帅帅.干旱胁迫对谷子生理特性的影响及赤霉素代谢关键酶基因表达分析[D].晋中:山西农业大学, 2014.
[34] Divi U K, Krishna P. Overexpression of the brassinosterid biosynthetic gene AtDWF4 in Arabidopsis and increases cold tolerance in transgenic seedlings[J]. Plant Growth Reg,2010,29(4):385-393.
[35] 杨作仁.棉花T-DNA激活标签突变体pag1分子机制的研究与应用[D].北京:中国农业科学院,2014.

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