Construction and Evaluation of cDNA Library of Flower and Fruit of Grapevine cv.″Summer Black″

doi:10.7668/hbnxb.2010.04.006

Abstract

Abstract: Grapevine is one economically important fruit crop in the world. Construction of the full-length cDNA library of grape flower and berry is the importantly fundamental work for the study on the molecular mechanism of the development of grape flower and berry，i n which it can facilitate the cloning，characterization and utilization of the important genes involved. In this research，a full-length cDNA library of flower and berry of grapevine cv . ″Summer Black″，a well known grape cultivar in its high popularity in cultivation and great berry quality，was constructed with Creator SMART cDNA Construction Kit. This library has a capacity of 1. 2 × 106 clones，and its recombination efficiency was about 99%，and the insert sizes were about 1. 0－3. 0 kb based on PCR analysis of 30 randomly chosen clones， suggesting that the cDNA library of grape flower and fruit has been successfully constructed for the use in further study.

Key words: Grapevine, Flower, Fruit, RNA extract, cDNA library

CLC Number:

WANG Chen, WANG Wen-yan, CHU Jian-qing, YANG Guang, GUO Lei, FANG Jing-gui. Construction and Evaluation of cDNA Library of Flower and Fruit of Grapevine cv.″Summer Black″[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 30-34. doi: 10.7668/hbnxb.2010.04.006.

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http://www.hbnxb.net/EN/Y2010/V25/I4/30

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