Cloning and Characterization of β(1,3;1,4)Beta Glucanase Gene from TcLr35 Wheat

doi:10.7668/hbnxb.2010.04.005

Abstract

Abstract: One pair of primer was designed based on the β(1，3; 1，4) beta glucanase，a full length wheat β(1，3;1，4) beta glucanase gene was obtained by RT-PCR and RACE from the RNA of TcLr35 carrying the Lr35 gene conferring resistance against wheat leaf rust induced by the Puccinia triticina named PR34，which was 1 416 bp in length， and included one complete open reading frame encoding 335 amino acids，a 379 bp 3' untranslated region(3' UTR) and 29 bp poly(A) tails. The initiation codon and stop codon were found in 13 bp locus and 1 015 bp locus respectively. The deduced amino acid sequence showed close homology to beta glucanase proteins which have been isolated from wheat， barley，and many other plants. The deduced amino acids contained Glyco_hydro_17 conserved domains which was related to beta glucanase. Southern blot indicated that the TcLr35 genome contained low copies of PR34 gene.

Key words: Wheat, β(1, 3, 1, 4) beta glucanase, Gene cloning, Sequence analysis

CLC Number:

Q78
S432.44

WANG Hai-yan, LIU Da-qun, YANG Wen-xiang, LI Zai-feng, ZHANG Li-rong, LI Xing. Cloning and Characterization of β(1,3;1,4)Beta Glucanase Gene from TcLr35 Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 25-29. doi: 10.7668/hbnxb.2010.04.005.

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References

[1] Komer J A.Genetics of resistance to wheat leaf rust[J].Annu Revi Phytopathology,1996,34:435-455.

[2] Denissen C J M.Influence of race and post infection temperature on two components of partial resistance to wheat leaf rust in seedlings of wheat[J].Euphytica,1991,58(1):13-20.

[3] Catherine F,Silvia T,Nils S.Map-based isolation of the leaf rust disease resistance gene Lr10 from the hexaploid wheat(Triticum aestivum L.)genome[J].Proc Natl Acad Sci USA,2003,100(25):15253-15258.

[4] Huang L,Steven A,Wanlong L,et al.Map-based cloning of leaf rust resistance gene Lr21 from the large and polyploid genome of bread wheat[J].Genetics,2003,164:655-664.

[5] Cloutier S,Brent D,Caroline McCallum.Leaf rust resistance gene Lr1,isolated from bread wheat(Triticum aestivum L.)is a member of the large psr567 gene family[J].Plant Mol Biol,2007,65:93-106.

[6] Krattinger S G,Lagudah E S,Spielmeyer W,et al.A multi-pathogen resistance QTL in wheat is controlled by a single gene[J].Science,2009,323:1360 -1363.

[7] Li W L,Faris J D,Chittoor J M,et al.Genomic mapping of defense response genes in wheat[J].Theo Appl Genet,1999,98:226-233.

[8] Arlorio M,Ludwig A,BollerT,et al.Inhibition of fungal growth by plant chitinases and β-1,3-glucanases:a morphological study[J].Pro-toplasma,1992,171:34-43.

[9] Doreen M L,Peter B H,Geoffrey B F.Purification and characterization of β-(1-3,-4)glucan endohydrolases from germinated wheat(Triticum aestivum)[J].Plant Molecular Biology,1993,22(5):847-859.

[10] Linthorst J M,Melchers L S,Mayers A,et al.Analysis of gene families encoding acidic and basic β-1,3-glucanases of tobacco[J].Proc Natl Acad Sci USA,1990,87:8756-8760.

[11] 郑用琏,Balance G M.小麦与褐斑病菌之间的分子互作Ⅰ:受病原菌诱导的寄主PR基因的表达[J].华中农业大学学报,1994,13(3):213-218.

[12] 邬珺超,蒋滢.cDNA末端快速扩增技术的研究进展[J].氨基酸和生物资源,2003,25(1):25-31.

[13] 邢全华,王广金,石金锋,等.β-1,3-葡聚糖酶基因高效表达载体的构建及对小麦的转化[J].遗传学报,2003,30(8):717-722.

[14] 王华忠,邢丽萍,陈佩度.利用瞬间表达技术分析小麦几丁质酶和β-1,3葡聚糖苷酶基因的抗病性功能[J].麦类作物学报,2006,26(1):7-12.

[15] 邢莉萍,王华忠,蒋正宁,等.小麦类甜蛋白基因的转化及转基因植株的抗病性分析[J].作物学报,2008,34(3):349-354.

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