Non-Promotor Gene Targeting Vector Construction for Bovine β-casein Site

doi:10.7668/hbnxb.2010.04.004

Abstract

Abstract: Plasmid vector pEGFP-C1，including neo and GFP gene，was modified. Psv40 promoter for neo was deleted，and introduce a LoxP locus into each flank of non-promoter neo and complete GFP，constructed pEGFPLoxP. Then we introduced 1 590 bp and 5 833 bp sequences from bovine genomes into vector pEGFP-LoxP，completed construction of non-promoter gene targeting vector，which is pT-1590-LoxP-GFP-LoxP-5833，to bovine β-casein gene locus.

Key words: Bovine β-casein, LoxP, Non-promoter, Targeting vector, Construction

CLC Number:

Q785

LI Rui-guo, MIAO Chao-hua, HOU Jian, GUAN Hong, AN Xiao-rong, CHEN Yong-fu. Non-Promotor Gene Targeting Vector Construction for Bovine β-casein Site[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 17-24. doi: 10.7668/hbnxb.2010.04.004.

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