Analysis of Genetic Diversity and Phylogeny of Five Chinese Subspecies of Wapiti Using Mitochondrial Cytb Complete Sequence

doi:10.7668/hbnxb.2010.04.003

Abstract

Abstract: To examine molecular genetic diversity and phylogeny of wapiti，mitochondrial Cytb complete sequence have been analyzed in 43 wapiti from 5 Chinese populations. The results showed that content of A，T，G and C in mitochondrial Cytb complete sequence were not significantly different among 5 populations. Diverse loci took 7. 63% of analyzed loci. For Tianshan wapiti and Talimu wapiti respectively，haplotypes ratios were 100% and 50% ; haplotypes diversity (Hd) were (1. 000 ± 0. 218) and (0. 700 ± 0. 096) ; nucleotide diversity (Pi) were 0. 003 33 and 0. 005 44; average number of nucleotide difference (K) were 3. 800 and 6. 200. It is suggested that the 5 populations are highly genetic diverse. Analysis on haplotypes phylogeny of 13 wapiti showed that there were 2 maternal origins in China. Comparison between Cytb haplotypes of wapiti in this study to those of other 33 wapiti obtained from GenBank has been done using Network relation analysis. The results showed that the Talimu wapiti was phylogenetically close to wapiti populations in the western countries.

Key words: Mitochondrial DNA, Cytochrome b, Genetic diversity, Phylogenetic, Wapiti

CLC Number:

ZHANG Li, GUN Shuang-bao, LEI Tian-yun, LIU Li-xia, QIN Da-wei, ZHAO Shi-feng. Analysis of Genetic Diversity and Phylogeny of Five Chinese Subspecies of Wapiti Using Mitochondrial Cytb Complete Sequence[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 12-16. doi: 10.7668/hbnxb.2010.04.003.

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